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The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus

Evoked activity in OSNs in I7-GFP WT and KO mice for TMEM16B. (A and B) Representative recordings of responses to a puff of 50 ms of 500 µM heptanal for two different OSNs from WT (A) and KO mice (B). The bottom panels show the regions enclosed by the dashed boxes on an expanded time scale. Broken red lines represent the onset of heptanal stimulation, and the red bars in the bottom panels its duration. (C and D) Raster plots on an enlarged time scale for the same cells shown in A and B. (E) Averaged responses to the same odorant puff as A and B, normalized to the number of neurons (n = 13 from 7 WT mice, n = 9 from 5 KO mice; bin 300 ms). The broken red line represents the onset of stimulation, and data belong to WT and KO PSTH distributions. *, 0.01 < P < 0.05; and **, P < 0.01. (F–H) Scatter plot of ISI of the first four spikes (F; 23 ± 13 ms in WT, 20 ± 11 ms in KO; mean ± SD; Mann-Whitney U test, one tail: P > 0.05), of the total number of spikes (G; 12 ± 10 spike/response in WT, 70 ± 57 spike/response in KO; Mann-Whitney U test, one tail: **, P < 0.01), and of the response duration (H; 2.3 ± 1.3 s in WT, 8.1 ± 7.0 s in KO; Mann-Whitney U test, one tail: **, P < 0.01). In the box plots the squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. The number of experiments is indicated in parentheses.
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fig6: Evoked activity in OSNs in I7-GFP WT and KO mice for TMEM16B. (A and B) Representative recordings of responses to a puff of 50 ms of 500 µM heptanal for two different OSNs from WT (A) and KO mice (B). The bottom panels show the regions enclosed by the dashed boxes on an expanded time scale. Broken red lines represent the onset of heptanal stimulation, and the red bars in the bottom panels its duration. (C and D) Raster plots on an enlarged time scale for the same cells shown in A and B. (E) Averaged responses to the same odorant puff as A and B, normalized to the number of neurons (n = 13 from 7 WT mice, n = 9 from 5 KO mice; bin 300 ms). The broken red line represents the onset of stimulation, and data belong to WT and KO PSTH distributions. *, 0.01 < P < 0.05; and **, P < 0.01. (F–H) Scatter plot of ISI of the first four spikes (F; 23 ± 13 ms in WT, 20 ± 11 ms in KO; mean ± SD; Mann-Whitney U test, one tail: P > 0.05), of the total number of spikes (G; 12 ± 10 spike/response in WT, 70 ± 57 spike/response in KO; Mann-Whitney U test, one tail: **, P < 0.01), and of the response duration (H; 2.3 ± 1.3 s in WT, 8.1 ± 7.0 s in KO; Mann-Whitney U test, one tail: **, P < 0.01). In the box plots the squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. The number of experiments is indicated in parentheses.

Mentions: Data in Figs. 5, 6, 7, and 8 are presented as mean value and boxplots in which the inner square represents the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. Spontaneous and evoked firing frequency data were not in all cases normally distributed (Kolmogorov-Smirnov test or Shapiro-Wilkinson test), and statistical significance was determined using Mann-Whitney U test. P-values <0.05 were considered statistically significant.


The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons
Evoked activity in OSNs in I7-GFP WT and KO mice for TMEM16B. (A and B) Representative recordings of responses to a puff of 50 ms of 500 µM heptanal for two different OSNs from WT (A) and KO mice (B). The bottom panels show the regions enclosed by the dashed boxes on an expanded time scale. Broken red lines represent the onset of heptanal stimulation, and the red bars in the bottom panels its duration. (C and D) Raster plots on an enlarged time scale for the same cells shown in A and B. (E) Averaged responses to the same odorant puff as A and B, normalized to the number of neurons (n = 13 from 7 WT mice, n = 9 from 5 KO mice; bin 300 ms). The broken red line represents the onset of stimulation, and data belong to WT and KO PSTH distributions. *, 0.01 < P < 0.05; and **, P < 0.01. (F–H) Scatter plot of ISI of the first four spikes (F; 23 ± 13 ms in WT, 20 ± 11 ms in KO; mean ± SD; Mann-Whitney U test, one tail: P > 0.05), of the total number of spikes (G; 12 ± 10 spike/response in WT, 70 ± 57 spike/response in KO; Mann-Whitney U test, one tail: **, P < 0.01), and of the response duration (H; 2.3 ± 1.3 s in WT, 8.1 ± 7.0 s in KO; Mann-Whitney U test, one tail: **, P < 0.01). In the box plots the squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. The number of experiments is indicated in parentheses.
© Copyright Policy - openaccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037344&req=5

fig6: Evoked activity in OSNs in I7-GFP WT and KO mice for TMEM16B. (A and B) Representative recordings of responses to a puff of 50 ms of 500 µM heptanal for two different OSNs from WT (A) and KO mice (B). The bottom panels show the regions enclosed by the dashed boxes on an expanded time scale. Broken red lines represent the onset of heptanal stimulation, and the red bars in the bottom panels its duration. (C and D) Raster plots on an enlarged time scale for the same cells shown in A and B. (E) Averaged responses to the same odorant puff as A and B, normalized to the number of neurons (n = 13 from 7 WT mice, n = 9 from 5 KO mice; bin 300 ms). The broken red line represents the onset of stimulation, and data belong to WT and KO PSTH distributions. *, 0.01 < P < 0.05; and **, P < 0.01. (F–H) Scatter plot of ISI of the first four spikes (F; 23 ± 13 ms in WT, 20 ± 11 ms in KO; mean ± SD; Mann-Whitney U test, one tail: P > 0.05), of the total number of spikes (G; 12 ± 10 spike/response in WT, 70 ± 57 spike/response in KO; Mann-Whitney U test, one tail: **, P < 0.01), and of the response duration (H; 2.3 ± 1.3 s in WT, 8.1 ± 7.0 s in KO; Mann-Whitney U test, one tail: **, P < 0.01). In the box plots the squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. The number of experiments is indicated in parentheses.
Mentions: Data in Figs. 5, 6, 7, and 8 are presented as mean value and boxplots in which the inner square represents the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. Spontaneous and evoked firing frequency data were not in all cases normally distributed (Kolmogorov-Smirnov test or Shapiro-Wilkinson test), and statistical significance was determined using Mann-Whitney U test. P-values <0.05 were considered statistically significant.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus