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The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons

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ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus

Evoked activity recorded with the suction pipette technique in OSNs from WT and KO mice. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX. Insets show responses on an expanded time scale. The recording bandwidth was 0–5,000 Hz to display APs. (C–F) Differences in the response were quantified for the number of spikes (C), the duration of the train of elicited spikes (D), the delay of the first spike (E), and maximal firing frequency (F). Mean ± SEM; number of experiments is indicated in parentheses. Unpaired t test: *, 0.01 < P < 0.05.
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fig4: Evoked activity recorded with the suction pipette technique in OSNs from WT and KO mice. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX. Insets show responses on an expanded time scale. The recording bandwidth was 0–5,000 Hz to display APs. (C–F) Differences in the response were quantified for the number of spikes (C), the duration of the train of elicited spikes (D), the delay of the first spike (E), and maximal firing frequency (F). Mean ± SEM; number of experiments is indicated in parentheses. Unpaired t test: *, 0.01 < P < 0.05.

Mentions: In a first set of experiments, we compared AP firing in isolated OSNs from WT and KO mice stimulated by 1-s application of 1 mM IBMX using suction electrode recordings. In this recording configuration, both transduction current and APs, which are generated as the voltage is free to vary, can be simultaneously recorded using a large bandwidth filter setting (see Materials and methods). In WT OSNs, we recorded AP firing during the early rising phase of the IBMX-stimulated transduction current (Fig. 4 A). Surprisingly, TMEM16B KO OSNs fired APs during the entire IBMX transduction current (Fig. 4 B). On average, the number of spikes was significantly higher, and the spike train duration (measured as the time from the first to the last spike of the train to occur) was significantly longer in TMEM16B KO compared with WT mice (Fig. 4, A and B [insets], C, and D). Other parameters, such as the delay of the first AP (Fig. 4 E) and the maximal firing frequency (Fig. 4 F) were not significantly different in KO and WT mice. These results show that the lack of CaCCs altered the firing behavior in response to IBMX by increasing the number of spikes and prolonging the duration of the spike train in isolated OSNs from TMEM16B KO mice.


The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons
Evoked activity recorded with the suction pipette technique in OSNs from WT and KO mice. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX. Insets show responses on an expanded time scale. The recording bandwidth was 0–5,000 Hz to display APs. (C–F) Differences in the response were quantified for the number of spikes (C), the duration of the train of elicited spikes (D), the delay of the first spike (E), and maximal firing frequency (F). Mean ± SEM; number of experiments is indicated in parentheses. Unpaired t test: *, 0.01 < P < 0.05.
© Copyright Policy - openaccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037344&req=5

fig4: Evoked activity recorded with the suction pipette technique in OSNs from WT and KO mice. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX. Insets show responses on an expanded time scale. The recording bandwidth was 0–5,000 Hz to display APs. (C–F) Differences in the response were quantified for the number of spikes (C), the duration of the train of elicited spikes (D), the delay of the first spike (E), and maximal firing frequency (F). Mean ± SEM; number of experiments is indicated in parentheses. Unpaired t test: *, 0.01 < P < 0.05.
Mentions: In a first set of experiments, we compared AP firing in isolated OSNs from WT and KO mice stimulated by 1-s application of 1 mM IBMX using suction electrode recordings. In this recording configuration, both transduction current and APs, which are generated as the voltage is free to vary, can be simultaneously recorded using a large bandwidth filter setting (see Materials and methods). In WT OSNs, we recorded AP firing during the early rising phase of the IBMX-stimulated transduction current (Fig. 4 A). Surprisingly, TMEM16B KO OSNs fired APs during the entire IBMX transduction current (Fig. 4 B). On average, the number of spikes was significantly higher, and the spike train duration (measured as the time from the first to the last spike of the train to occur) was significantly longer in TMEM16B KO compared with WT mice (Fig. 4, A and B [insets], C, and D). Other parameters, such as the delay of the first AP (Fig. 4 E) and the maximal firing frequency (Fig. 4 F) were not significantly different in KO and WT mice. These results show that the lack of CaCCs altered the firing behavior in response to IBMX by increasing the number of spikes and prolonging the duration of the spike train in isolated OSNs from TMEM16B KO mice.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus