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The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons

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ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus

Transduction current recorded with the suction pipette technique from isolated OSNs. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX in Ringer’s solution and in the presence or absence of 300 µM NFA, a Cl− channel blocker. (C and D) Suction peak currents measured in control, in the presence of NFA and after washout (C) and ratio of the response in the presence and absence of NFA (D). Mean ± SEM, n = 6 for WT and KO. Unpaired t test: **, P < 0.01.
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fig3: Transduction current recorded with the suction pipette technique from isolated OSNs. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX in Ringer’s solution and in the presence or absence of 300 µM NFA, a Cl− channel blocker. (C and D) Suction peak currents measured in control, in the presence of NFA and after washout (C) and ratio of the response in the presence and absence of NFA (D). Mean ± SEM, n = 6 for WT and KO. Unpaired t test: **, P < 0.01.

Mentions: We also investigated the contribution of CaCCs to stimulus-induced responses using the suction pipette technique in isolated OSNs (Fig. 3). We stimulated OSNs with the phosphodiesterase inhibitor IBMX, a feasible alternative to odorant stimulation, as OSNs can have a high intrinsic activity (Reisert et al., 2007). Currents elicited by 1 mM IBMX (1-s application) were greatly reduced by application of the Cl− current blocker NFA (300 µM) in WT OSNs (Fig. 3, A, C, and D). In OSNs from TMEM16B KO mice, IBMX application produced a significantly smaller current in KO compared with WT mice (Fig. 3, B and C). This small current could not be further reduced by the application of NFA (Fig. 3, B–D), a result consistent with the presence of a residual CNG current.


The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons
Transduction current recorded with the suction pipette technique from isolated OSNs. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX in Ringer’s solution and in the presence or absence of 300 µM NFA, a Cl− channel blocker. (C and D) Suction peak currents measured in control, in the presence of NFA and after washout (C) and ratio of the response in the presence and absence of NFA (D). Mean ± SEM, n = 6 for WT and KO. Unpaired t test: **, P < 0.01.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5037344&req=5

fig3: Transduction current recorded with the suction pipette technique from isolated OSNs. (A and B) Single-cell suction electrode recordings from a dissociated WT (A) and KO (B) OSN stimulated with a 1-s pulse of 1 mM IBMX in Ringer’s solution and in the presence or absence of 300 µM NFA, a Cl− channel blocker. (C and D) Suction peak currents measured in control, in the presence of NFA and after washout (C) and ratio of the response in the presence and absence of NFA (D). Mean ± SEM, n = 6 for WT and KO. Unpaired t test: **, P < 0.01.
Mentions: We also investigated the contribution of CaCCs to stimulus-induced responses using the suction pipette technique in isolated OSNs (Fig. 3). We stimulated OSNs with the phosphodiesterase inhibitor IBMX, a feasible alternative to odorant stimulation, as OSNs can have a high intrinsic activity (Reisert et al., 2007). Currents elicited by 1 mM IBMX (1-s application) were greatly reduced by application of the Cl− current blocker NFA (300 µM) in WT OSNs (Fig. 3, A, C, and D). In OSNs from TMEM16B KO mice, IBMX application produced a significantly smaller current in KO compared with WT mice (Fig. 3, B and C). This small current could not be further reduced by the application of NFA (Fig. 3, B–D), a result consistent with the presence of a residual CNG current.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus