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The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons

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ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus

Behavioral deficits in TMEM16B KO mice. (A) WT and KO mice had to locate an Oreo cookie buried in the bedding of their cage. Experiments were performed once a day over 5 d, and on the sixth day the cookie was placed on top of the bedding. (B) After 1 wk on normal diet, the same mice were exposed to a series of odors that were known (Oreo and chow) or novel (peanut, cheese, or chocolate) to them. Mann-Whitney U test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Mean ± SEM from 11 WT and 13 KO mice.
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fig1: Behavioral deficits in TMEM16B KO mice. (A) WT and KO mice had to locate an Oreo cookie buried in the bedding of their cage. Experiments were performed once a day over 5 d, and on the sixth day the cookie was placed on top of the bedding. (B) After 1 wk on normal diet, the same mice were exposed to a series of odors that were known (Oreo and chow) or novel (peanut, cheese, or chocolate) to them. Mann-Whitney U test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Mean ± SEM from 11 WT and 13 KO mice.

Mentions: We used an odor-guided food-seeking test where mice have to use their olfactory ability to locate a food item buried under the bedding chips in the cage. We ran the test for a consecutive 5 d. On the first day of testing, when mice were naive with respect to the food item and its odor (a piece of Oreo cookie), we observed that close to a fourth of TMEM16B KO mice (3 out of 13) failed to locate the food within the 10-min test time, whereas none of the WT exceeded 10 min. On average, KO mice were significantly slower in locating the cookie (Fig. 1 A; Mann-Whitney U test, P < 0.05). During the following 4 d of testing, both WT and KO mice began to locate the food faster, and in the end, KO mice could perform the task as quickly as WT. TMEM16B KO mice did not show any gross motor, metabolic, or motivational deficits as they performed equally well as WT mice when the food was presented visually on top of the bedding and not buried underneath it (Fig. 1 A).


The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons
Behavioral deficits in TMEM16B KO mice. (A) WT and KO mice had to locate an Oreo cookie buried in the bedding of their cage. Experiments were performed once a day over 5 d, and on the sixth day the cookie was placed on top of the bedding. (B) After 1 wk on normal diet, the same mice were exposed to a series of odors that were known (Oreo and chow) or novel (peanut, cheese, or chocolate) to them. Mann-Whitney U test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Mean ± SEM from 11 WT and 13 KO mice.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5037344&req=5

fig1: Behavioral deficits in TMEM16B KO mice. (A) WT and KO mice had to locate an Oreo cookie buried in the bedding of their cage. Experiments were performed once a day over 5 d, and on the sixth day the cookie was placed on top of the bedding. (B) After 1 wk on normal diet, the same mice were exposed to a series of odors that were known (Oreo and chow) or novel (peanut, cheese, or chocolate) to them. Mann-Whitney U test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. Mean ± SEM from 11 WT and 13 KO mice.
Mentions: We used an odor-guided food-seeking test where mice have to use their olfactory ability to locate a food item buried under the bedding chips in the cage. We ran the test for a consecutive 5 d. On the first day of testing, when mice were naive with respect to the food item and its odor (a piece of Oreo cookie), we observed that close to a fourth of TMEM16B KO mice (3 out of 13) failed to locate the food within the 10-min test time, whereas none of the WT exceeded 10 min. On average, KO mice were significantly slower in locating the cookie (Fig. 1 A; Mann-Whitney U test, P < 0.05). During the following 4 d of testing, both WT and KO mice began to locate the food faster, and in the end, KO mice could perform the task as quickly as WT. TMEM16B KO mice did not show any gross motor, metabolic, or motivational deficits as they performed equally well as WT mice when the food was presented visually on top of the bedding and not buried underneath it (Fig. 1 A).

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Related in: MedlinePlus