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Fibrinogen-thrombin collagen patch reinforcement of high-risk colonic anastomoses in rats

View Article: PubMed Central - PubMed

ABSTRACT

Aim: To evaluate the effectiveness of human fibrinogen-thrombin collagen patch (TachoSil®) in the reinforcement of high-risk colon anastomoses.

Methods: A quasi-experimental study was conducted in Wistar rats (n = 56) that all underwent high-risk anastomoses (anastomosis with only two sutures) after colectomies. The rats were divided into two randomized groups: Control group (24 rats) and treatment group (24 rats). In the treatment group, high-risk anastomosis was reinforced with TachoSil® (a piece of TachoSil® was applied over this high-risk anastomosis, covering the gap). Leak incidence, overall survival, intra-abdominal adhesions, and histologic healing of anastomoses were analyzed. Survivors were divided into two subgroups and euthanized at 15 and 30 d after intervention in order to analyze the adhesions and histologic changes.

Results: Overall survival was 71.4% and 57.14% in the TachoSil® group and control group, respectively (P = 0.29); four rats died from other causes and six rats in the treatment group and 10 in the control group experienced colonic leakage (P > 0.05). The intra-abdominal adhesion score was similar in both groups, with no differences between subgroups. We found non-significant differences in the healing process according to the histologic score used in both groups (P = 0.066).

Conclusion: In our study, the use of TachoSil® was associated with a non-statistically significant reduction in the rate of leakage in high-risk anastomoses. TachoSil® has been shown to be a safe product because it does not affect the histologic healing process or increase intra-abdominal adhesions.

No MeSH data available.


Survival in both groups.
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Figure 2: Survival in both groups.

Mentions: The number of events, defined as death as a consequence of colonic leakage, was 10 (35.7%) in the control group and 6 (21.4%) in the TachoSil® group. All deaths happened before the fourth post-operative day. Four animals in both groups died due to other causes, namely hemorrhage, post-anesthesia and bowel obstruction, with no relation to the experimental study. The mean survival per group was 19.5 ± 2.6 (control) and 23.7 ± 2.2 d (TachoSil®). With these results, the overall survival was 57.14% and 71.4% in the control and TachoSil® groups, respectively (P = 0.29) (Figure 2), with no significant differences between the groups.


Fibrinogen-thrombin collagen patch reinforcement of high-risk colonic anastomoses in rats
Survival in both groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037336&req=5

Figure 2: Survival in both groups.
Mentions: The number of events, defined as death as a consequence of colonic leakage, was 10 (35.7%) in the control group and 6 (21.4%) in the TachoSil® group. All deaths happened before the fourth post-operative day. Four animals in both groups died due to other causes, namely hemorrhage, post-anesthesia and bowel obstruction, with no relation to the experimental study. The mean survival per group was 19.5 ± 2.6 (control) and 23.7 ± 2.2 d (TachoSil®). With these results, the overall survival was 57.14% and 71.4% in the control and TachoSil® groups, respectively (P = 0.29) (Figure 2), with no significant differences between the groups.

View Article: PubMed Central - PubMed

ABSTRACT

Aim: To evaluate the effectiveness of human fibrinogen-thrombin collagen patch (TachoSil®) in the reinforcement of high-risk colon anastomoses.

Methods: A quasi-experimental study was conducted in Wistar rats (n = 56) that all underwent high-risk anastomoses (anastomosis with only two sutures) after colectomies. The rats were divided into two randomized groups: Control group (24 rats) and treatment group (24 rats). In the treatment group, high-risk anastomosis was reinforced with TachoSil® (a piece of TachoSil® was applied over this high-risk anastomosis, covering the gap). Leak incidence, overall survival, intra-abdominal adhesions, and histologic healing of anastomoses were analyzed. Survivors were divided into two subgroups and euthanized at 15 and 30 d after intervention in order to analyze the adhesions and histologic changes.

Results: Overall survival was 71.4% and 57.14% in the TachoSil® group and control group, respectively (P = 0.29); four rats died from other causes and six rats in the treatment group and 10 in the control group experienced colonic leakage (P > 0.05). The intra-abdominal adhesion score was similar in both groups, with no differences between subgroups. We found non-significant differences in the healing process according to the histologic score used in both groups (P = 0.066).

Conclusion: In our study, the use of TachoSil® was associated with a non-statistically significant reduction in the rate of leakage in high-risk anastomoses. TachoSil® has been shown to be a safe product because it does not affect the histologic healing process or increase intra-abdominal adhesions.

No MeSH data available.