Limits...
Mycobacterium bovis infection in a wild sow ( Sus scrofa ): the first case in Korea

View Article: PubMed Central - PubMed

ABSTRACT

Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries. We report here the first case of M. bovis infection in a domesticated wild sow in Korea. Granulomatous and necrotizing lesions with small numbers of acid-fast bacilli were observed in nodules of the lung of wild sow. Furthermore, the M. bovis isolate from the wild sow had spoligotype SB0140 and a novel MIRU-VNTR allelic profile, which is not found in cattle and deer in Korea.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis of the polymerase chain reaction products using primers for the rpoB gene (518 bp), RD8 (Mycobacterium [M.] bovis and M. bovis BCG, 360 bp; M. tuberculosis, 150 bp), and RD1 (254 bp). Lane M, 100-bp DNA size marker; Lane 1, wild boar isolate; Lane 2, M. bovis AN5; Lane 3, M. bovis BCG Pasteur; Lane 4, M. tuberculosis H37Rv.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037313&req=5

Figure 2: Agarose gel electrophoresis of the polymerase chain reaction products using primers for the rpoB gene (518 bp), RD8 (Mycobacterium [M.] bovis and M. bovis BCG, 360 bp; M. tuberculosis, 150 bp), and RD1 (254 bp). Lane M, 100-bp DNA size marker; Lane 1, wild boar isolate; Lane 2, M. bovis AN5; Lane 3, M. bovis BCG Pasteur; Lane 4, M. tuberculosis H37Rv.

Mentions: M. bovis was isolated and identified using multiplex PCR assay (Fig. 2). The spoligotype was determined as SB0140, and the MIRU-VNTR allelic profile was 253264103433101 based on the MIRU 2, MIRU 26, MIRU 27, MIRU 31, ETR-A, ETR-B, QUB 11a, QUB 18, QUB 26, VNTR 2401, VNTR 3171, VNTR 3232, and VNTR 3336 loci.


Mycobacterium bovis infection in a wild sow ( Sus scrofa ): the first case in Korea
Agarose gel electrophoresis of the polymerase chain reaction products using primers for the rpoB gene (518 bp), RD8 (Mycobacterium [M.] bovis and M. bovis BCG, 360 bp; M. tuberculosis, 150 bp), and RD1 (254 bp). Lane M, 100-bp DNA size marker; Lane 1, wild boar isolate; Lane 2, M. bovis AN5; Lane 3, M. bovis BCG Pasteur; Lane 4, M. tuberculosis H37Rv.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037313&req=5

Figure 2: Agarose gel electrophoresis of the polymerase chain reaction products using primers for the rpoB gene (518 bp), RD8 (Mycobacterium [M.] bovis and M. bovis BCG, 360 bp; M. tuberculosis, 150 bp), and RD1 (254 bp). Lane M, 100-bp DNA size marker; Lane 1, wild boar isolate; Lane 2, M. bovis AN5; Lane 3, M. bovis BCG Pasteur; Lane 4, M. tuberculosis H37Rv.
Mentions: M. bovis was isolated and identified using multiplex PCR assay (Fig. 2). The spoligotype was determined as SB0140, and the MIRU-VNTR allelic profile was 253264103433101 based on the MIRU 2, MIRU 26, MIRU 27, MIRU 31, ETR-A, ETR-B, QUB 11a, QUB 18, QUB 26, VNTR 2401, VNTR 3171, VNTR 3232, and VNTR 3336 loci.

View Article: PubMed Central - PubMed

ABSTRACT

Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries. We report here the first case of M. bovis infection in a domesticated wild sow in Korea. Granulomatous and necrotizing lesions with small numbers of acid-fast bacilli were observed in nodules of the lung of wild sow. Furthermore, the M. bovis isolate from the wild sow had spoligotype SB0140 and a novel MIRU-VNTR allelic profile, which is not found in cattle and deer in Korea.

No MeSH data available.


Related in: MedlinePlus