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Reproductive ability of a cloned male detector dog and behavioral traits of its offspring

View Article: PubMed Central - PubMed

ABSTRACT

In 2007, seven detector dogs were produced by somatic cell nuclear transfer using one nuclear donor dog, then trained and certified as excellent detector dogs, similar to their donor. In 2011, we crossed a cloned male and normal female by natural breeding and produced ten offspring. In this study, we investigated the puppies' temperaments, which we later compared with those of the cloned parent male. The results show that the cloned male had normal reproductive abilities and produced healthy offspring. All puppies completed narcotic detector dog training with a success rate for selection of 60%. Although the litter of cloned males was small in this study, a cloned male dog bred by natural mating produced puppies that later successfully completed the training course for drug detection. In conclusion, cloning an elite dog with superior genetic factors and breeding of the cloned dog was found to be a useful method to efficiently procure detector dogs.

No MeSH data available.


Birth of puppies. (A) Hormonal change in a female dog from estrus to delivery. The square indicates the fertile period of a female dog. While in these periods, she stayed with a male cloned dog in a room. The arrow indicates the delivery time. (B) Appearance of puppies at 1 day after delivery.
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Figure 1: Birth of puppies. (A) Hormonal change in a female dog from estrus to delivery. The square indicates the fertile period of a female dog. While in these periods, she stayed with a male cloned dog in a room. The arrow indicates the delivery time. (B) Appearance of puppies at 1 day after delivery.

Mentions: Natural breeding was performed to evaluate the fertility of the cloned male dog. At 61 days after mating, ten offspring (male: 5, female: 5) were born by natural delivery (Table 1; Fig. 1). The puppies were healthy, had a yellow coat color, and no abnormalities in morphology (Table 1; Fig. 2).


Reproductive ability of a cloned male detector dog and behavioral traits of its offspring
Birth of puppies. (A) Hormonal change in a female dog from estrus to delivery. The square indicates the fertile period of a female dog. While in these periods, she stayed with a male cloned dog in a room. The arrow indicates the delivery time. (B) Appearance of puppies at 1 day after delivery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037310&req=5

Figure 1: Birth of puppies. (A) Hormonal change in a female dog from estrus to delivery. The square indicates the fertile period of a female dog. While in these periods, she stayed with a male cloned dog in a room. The arrow indicates the delivery time. (B) Appearance of puppies at 1 day after delivery.
Mentions: Natural breeding was performed to evaluate the fertility of the cloned male dog. At 61 days after mating, ten offspring (male: 5, female: 5) were born by natural delivery (Table 1; Fig. 1). The puppies were healthy, had a yellow coat color, and no abnormalities in morphology (Table 1; Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

In 2007, seven detector dogs were produced by somatic cell nuclear transfer using one nuclear donor dog, then trained and certified as excellent detector dogs, similar to their donor. In 2011, we crossed a cloned male and normal female by natural breeding and produced ten offspring. In this study, we investigated the puppies' temperaments, which we later compared with those of the cloned parent male. The results show that the cloned male had normal reproductive abilities and produced healthy offspring. All puppies completed narcotic detector dog training with a success rate for selection of 60%. Although the litter of cloned males was small in this study, a cloned male dog bred by natural mating produced puppies that later successfully completed the training course for drug detection. In conclusion, cloning an elite dog with superior genetic factors and breeding of the cloned dog was found to be a useful method to efficiently procure detector dogs.

No MeSH data available.