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Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression

View Article: PubMed Central - PubMed

ABSTRACT

Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdcscid/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.

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SNP mammary gland tumor from subcutaneous tumor tissue, NOD.CB17-Prkdcscid/J mice. The circumscribed neoplastic nodules are located in the dermis and subcutaneous tissue. The tumor is composed of luminal epithelium-like cells. The neoplastic cells are arranged in a tubulopapillary fashion (arrows). The nuclei are round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders are distinct. Mitotic figures and pyknotic nuclei are common. Immunohistochemistry, positive signals for vimentin are scattered in the cytoplasm of the neoplastic cells. H&E stain. Scale bars = 500 µm (A), 50 µm (B and C).
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Figure 2: SNP mammary gland tumor from subcutaneous tumor tissue, NOD.CB17-Prkdcscid/J mice. The circumscribed neoplastic nodules are located in the dermis and subcutaneous tissue. The tumor is composed of luminal epithelium-like cells. The neoplastic cells are arranged in a tubulopapillary fashion (arrows). The nuclei are round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders are distinct. Mitotic figures and pyknotic nuclei are common. Immunohistochemistry, positive signals for vimentin are scattered in the cytoplasm of the neoplastic cells. H&E stain. Scale bars = 500 µm (A), 50 µm (B and C).

Mentions: A tumor formed in each mouse at the site of subcutaneous SNP cell injection. Histologically, the circumscribed neoplastic nodules were located in the dermis and subcutaneous tissue. The tumor was composed of luminal epithelium-like cells, and the neoplastic cells were arranged with a tubulopapillary architecture. The nuclei were round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders were distinct. Mitotic figures and pyknotic nuclei were observed for a large proportion of the cells (Fig. 2). Histopathologically, the morphological features of SNP cells were similar to those seen in the original CMGT cells. Some of the neoplastic cells showed positive signals for vimentin (panel C in Fig. 2). These findings were marked in the marginal region of the mass, as well as in the neoplastic cells within the vessels.


Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression
SNP mammary gland tumor from subcutaneous tumor tissue, NOD.CB17-Prkdcscid/J mice. The circumscribed neoplastic nodules are located in the dermis and subcutaneous tissue. The tumor is composed of luminal epithelium-like cells. The neoplastic cells are arranged in a tubulopapillary fashion (arrows). The nuclei are round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders are distinct. Mitotic figures and pyknotic nuclei are common. Immunohistochemistry, positive signals for vimentin are scattered in the cytoplasm of the neoplastic cells. H&E stain. Scale bars = 500 µm (A), 50 µm (B and C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037307&req=5

Figure 2: SNP mammary gland tumor from subcutaneous tumor tissue, NOD.CB17-Prkdcscid/J mice. The circumscribed neoplastic nodules are located in the dermis and subcutaneous tissue. The tumor is composed of luminal epithelium-like cells. The neoplastic cells are arranged in a tubulopapillary fashion (arrows). The nuclei are round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders are distinct. Mitotic figures and pyknotic nuclei are common. Immunohistochemistry, positive signals for vimentin are scattered in the cytoplasm of the neoplastic cells. H&E stain. Scale bars = 500 µm (A), 50 µm (B and C).
Mentions: A tumor formed in each mouse at the site of subcutaneous SNP cell injection. Histologically, the circumscribed neoplastic nodules were located in the dermis and subcutaneous tissue. The tumor was composed of luminal epithelium-like cells, and the neoplastic cells were arranged with a tubulopapillary architecture. The nuclei were round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders were distinct. Mitotic figures and pyknotic nuclei were observed for a large proportion of the cells (Fig. 2). Histopathologically, the morphological features of SNP cells were similar to those seen in the original CMGT cells. Some of the neoplastic cells showed positive signals for vimentin (panel C in Fig. 2). These findings were marked in the marginal region of the mass, as well as in the neoplastic cells within the vessels.

View Article: PubMed Central - PubMed

ABSTRACT

Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdcscid/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.

No MeSH data available.


Related in: MedlinePlus