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Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression

View Article: PubMed Central - PubMed

ABSTRACT

Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdcscid/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.

No MeSH data available.


Related in: MedlinePlus

Canine mammary gland tumor from mammary tissue, mixed breed dog. Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern (arrows) in the expanded milk ducts. Irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells were observed. Extensive necrosis is scattered throughout the neoplastic tissue. H&E stain. Scale bars = 200 µm (A), 50 µm (B).
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Figure 1: Canine mammary gland tumor from mammary tissue, mixed breed dog. Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern (arrows) in the expanded milk ducts. Irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells were observed. Extensive necrosis is scattered throughout the neoplastic tissue. H&E stain. Scale bars = 200 µm (A), 50 µm (B).

Mentions: Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern in the expanded milk ducts. The cells were characterized by irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells. In addition, extensive necrosis was scattered throughout the neoplastic tissue (Fig. 1). Based on these histopathological findings, the tumor was diagnosed as a CMGT.


Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression
Canine mammary gland tumor from mammary tissue, mixed breed dog. Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern (arrows) in the expanded milk ducts. Irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells were observed. Extensive necrosis is scattered throughout the neoplastic tissue. H&E stain. Scale bars = 200 µm (A), 50 µm (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037307&req=5

Figure 1: Canine mammary gland tumor from mammary tissue, mixed breed dog. Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern (arrows) in the expanded milk ducts. Irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells were observed. Extensive necrosis is scattered throughout the neoplastic tissue. H&E stain. Scale bars = 200 µm (A), 50 µm (B).
Mentions: Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern in the expanded milk ducts. The cells were characterized by irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells. In addition, extensive necrosis was scattered throughout the neoplastic tissue (Fig. 1). Based on these histopathological findings, the tumor was diagnosed as a CMGT.

View Article: PubMed Central - PubMed

ABSTRACT

Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdcscid/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.

No MeSH data available.


Related in: MedlinePlus