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Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013 – 2014

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ABSTRACT

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.

No MeSH data available.


Phylogenetic trees for gE (A), gI (B), gD (C) and PK (D) genes. Only bootstrap values greater than 50 are shown. Strains marked with black diamonds were isolated in our laboratory during 2013–2014.
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Figure 2: Phylogenetic trees for gE (A), gI (B), gD (C) and PK (D) genes. Only bootstrap values greater than 50 are shown. Strains marked with black diamonds were isolated in our laboratory during 2013–2014.

Mentions: To determine the phylogenetic relationships between the PRV circulating in southern China from 2013 to 2014 and other strains, phylogenetic trees were constructed based on the major viral genes (gE, TK, gI, PK, gD, 11K and 28K). Phylogenetic analysis of multiple sequences generated trees with similar profiles for all genes except PK and gD, which were classified in two primary clusters. Cluster 1 comprised strains from eastern countries (i.e., China, South Korea and Malaysia) exclusively, while cluster 2 represented strains from western countries and a small number of other Asian strains. Based on the genes involved in this study, PRV strains isolated in China from 2012 to 2014 were clustered into one group and also formed a relatively independent branch. In addition, PRV strain TJ (China, 2012) exhibited a marked genetic relationship with the strains under investigation (Figs. 2 and 3).


Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013 – 2014
Phylogenetic trees for gE (A), gI (B), gD (C) and PK (D) genes. Only bootstrap values greater than 50 are shown. Strains marked with black diamonds were isolated in our laboratory during 2013–2014.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037305&req=5

Figure 2: Phylogenetic trees for gE (A), gI (B), gD (C) and PK (D) genes. Only bootstrap values greater than 50 are shown. Strains marked with black diamonds were isolated in our laboratory during 2013–2014.
Mentions: To determine the phylogenetic relationships between the PRV circulating in southern China from 2013 to 2014 and other strains, phylogenetic trees were constructed based on the major viral genes (gE, TK, gI, PK, gD, 11K and 28K). Phylogenetic analysis of multiple sequences generated trees with similar profiles for all genes except PK and gD, which were classified in two primary clusters. Cluster 1 comprised strains from eastern countries (i.e., China, South Korea and Malaysia) exclusively, while cluster 2 represented strains from western countries and a small number of other Asian strains. Based on the genes involved in this study, PRV strains isolated in China from 2012 to 2014 were clustered into one group and also formed a relatively independent branch. In addition, PRV strain TJ (China, 2012) exhibited a marked genetic relationship with the strains under investigation (Figs. 2 and 3).

View Article: PubMed Central - PubMed

ABSTRACT

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.

No MeSH data available.