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Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013 – 2014

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ABSTRACT

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.

No MeSH data available.


Related in: MedlinePlus

Alignment of gE (A), PK (B), gI (C) and gD (D) genes of pseudorabies virus at the nucleotide level. Black box indicates the region of insertion or deletion.
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Figure 1: Alignment of gE (A), PK (B), gI (C) and gD (D) genes of pseudorabies virus at the nucleotide level. Black box indicates the region of insertion or deletion.

Mentions: Alignment of nucleotide sequences was conducted to identify differences between strains isolated in the Western and Eastern regions on a global scale. When compared with Kaplan and Becker, there were two insertions of six discontinuous nucleotides at positions 142–144 and 1,488–1,490 in the gE gene of the strains under investigation, which was similar to other strains isolated from China during 2012–2013 (panel A in Fig. 1). There was a 6 nt deletion at position 82–87 in the PK gene of the tested strains compared with Becker (panel B in Fig. 1). When compared with Kaplan and Becker, there was a 3 nt insertion at position 737–739 and a 3 nt deletion at position 518–520 in the gI gene of the tested strains (panel C in Fig. 1). A 6 nt insertion was identified in the gD gene of our isolated strains at nucleotide position 831–836 compared with Kaplan and Bartha (panel D in Fig. 1). The rest of the genes investigated in this study had no nucleotide insertions or deletions in common with the other PRV isolates.


Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013 – 2014
Alignment of gE (A), PK (B), gI (C) and gD (D) genes of pseudorabies virus at the nucleotide level. Black box indicates the region of insertion or deletion.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037305&req=5

Figure 1: Alignment of gE (A), PK (B), gI (C) and gD (D) genes of pseudorabies virus at the nucleotide level. Black box indicates the region of insertion or deletion.
Mentions: Alignment of nucleotide sequences was conducted to identify differences between strains isolated in the Western and Eastern regions on a global scale. When compared with Kaplan and Becker, there were two insertions of six discontinuous nucleotides at positions 142–144 and 1,488–1,490 in the gE gene of the strains under investigation, which was similar to other strains isolated from China during 2012–2013 (panel A in Fig. 1). There was a 6 nt deletion at position 82–87 in the PK gene of the tested strains compared with Becker (panel B in Fig. 1). When compared with Kaplan and Becker, there was a 3 nt insertion at position 737–739 and a 3 nt deletion at position 518–520 in the gI gene of the tested strains (panel C in Fig. 1). A 6 nt insertion was identified in the gD gene of our isolated strains at nucleotide position 831–836 compared with Kaplan and Bartha (panel D in Fig. 1). The rest of the genes investigated in this study had no nucleotide insertions or deletions in common with the other PRV isolates.

View Article: PubMed Central - PubMed

ABSTRACT

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.

No MeSH data available.


Related in: MedlinePlus