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Deep anterior lamellar keratoplasty of dog eyes using the big-bubble technique

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ABSTRACT

This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 µm, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet's membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7 days and 2% cyclosporine ointment every 12 h for 1 month. The eyes were examined post-operatively at 7, 14, 21, 28 and 150 days. The central portion of the transplanted cornea stayed transparent while corneal haze developed around the transplanted margin. Menace response was normal even though the transplanted cornea was edematous until 3 weeks after surgery. A marginal haze was rarely observed between the donor and recipient corneas at 150 days after the operation. A spotted haze developed in the central part of the deep stroma near the DM. Upon histopathological examination, the stroma and epithelium of the donor cornea had normal structures. Corneal transplantation using DALK with BBT can be performed in dogs preserving the healthy endothelium.

No MeSH data available.


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Histopathological evaluation with hematoxylin and eosin staining at 150 days after surgery. (A) The epithelium was overgrown (arrowhead) at the junction of the donor and recipient corneas. In addition, deformed stromal structures were detected at the transplant junction (black arrow). (B) The central corneal part had a normal corneal structure. A small piece of DM was left in the stroma (white arrow).
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Figure 4: Histopathological evaluation with hematoxylin and eosin staining at 150 days after surgery. (A) The epithelium was overgrown (arrowhead) at the junction of the donor and recipient corneas. In addition, deformed stromal structures were detected at the transplant junction (black arrow). (B) The central corneal part had a normal corneal structure. A small piece of DM was left in the stroma (white arrow).

Mentions: Upon histopathological examination, the stroma and epithelium of the donor cornea had normal structures (Fig. 4). A modified arrangement of the corneal stroma was confirmed in the junction between the donor and recipient corneas. In addition, keratocyte and epithelial proliferation and a distortion in the stromal structure were detected at the junction of the transplant. There were more severe pathological changes in the recipient cornea than in the donor parts. Some particles of the donor's DM in the cornea were observed in the stroma layer (Fig. 4).


Deep anterior lamellar keratoplasty of dog eyes using the big-bubble technique
Histopathological evaluation with hematoxylin and eosin staining at 150 days after surgery. (A) The epithelium was overgrown (arrowhead) at the junction of the donor and recipient corneas. In addition, deformed stromal structures were detected at the transplant junction (black arrow). (B) The central corneal part had a normal corneal structure. A small piece of DM was left in the stroma (white arrow).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037302&req=5

Figure 4: Histopathological evaluation with hematoxylin and eosin staining at 150 days after surgery. (A) The epithelium was overgrown (arrowhead) at the junction of the donor and recipient corneas. In addition, deformed stromal structures were detected at the transplant junction (black arrow). (B) The central corneal part had a normal corneal structure. A small piece of DM was left in the stroma (white arrow).
Mentions: Upon histopathological examination, the stroma and epithelium of the donor cornea had normal structures (Fig. 4). A modified arrangement of the corneal stroma was confirmed in the junction between the donor and recipient corneas. In addition, keratocyte and epithelial proliferation and a distortion in the stromal structure were detected at the junction of the transplant. There were more severe pathological changes in the recipient cornea than in the donor parts. Some particles of the donor's DM in the cornea were observed in the stroma layer (Fig. 4).

View Article: PubMed Central - PubMed

ABSTRACT

This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 µm, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet's membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7 days and 2% cyclosporine ointment every 12 h for 1 month. The eyes were examined post-operatively at 7, 14, 21, 28 and 150 days. The central portion of the transplanted cornea stayed transparent while corneal haze developed around the transplanted margin. Menace response was normal even though the transplanted cornea was edematous until 3 weeks after surgery. A marginal haze was rarely observed between the donor and recipient corneas at 150 days after the operation. A spotted haze developed in the central part of the deep stroma near the DM. Upon histopathological examination, the stroma and epithelium of the donor cornea had normal structures. Corneal transplantation using DALK with BBT can be performed in dogs preserving the healthy endothelium.

No MeSH data available.


Related in: MedlinePlus