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Deep anterior lamellar keratoplasty of dog eyes using the big-bubble technique

View Article: PubMed Central - PubMed

ABSTRACT

This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 µm, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet's membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7 days and 2% cyclosporine ointment every 12 h for 1 month. The eyes were examined post-operatively at 7, 14, 21, 28 and 150 days. The central portion of the transplanted cornea stayed transparent while corneal haze developed around the transplanted margin. Menace response was normal even though the transplanted cornea was edematous until 3 weeks after surgery. A marginal haze was rarely observed between the donor and recipient corneas at 150 days after the operation. A spotted haze developed in the central part of the deep stroma near the DM. Upon histopathological examination, the stroma and epithelium of the donor cornea had normal structures. Corneal transplantation using DALK with BBT can be performed in dogs preserving the healthy endothelium.

No MeSH data available.


Related in: MedlinePlus

Slit-lamp biomicroscopy with direct diffuse illumination of the corneas under 10× magnification. (A) 12 h after surgery, (B) 7 days, (C) 14 days, (D) 21 days, (E) 28 days and (F) 150 days after surgery.
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Figure 2: Slit-lamp biomicroscopy with direct diffuse illumination of the corneas under 10× magnification. (A) 12 h after surgery, (B) 7 days, (C) 14 days, (D) 21 days, (E) 28 days and (F) 150 days after surgery.

Mentions: A corneal haze was developed from 7 days after surgery at the central transplanted cornea and around the suture line (Table 1). The corneal haze of central corneal part was reduced from 21 days after surgery. However, the corneal haze around the junction of the donor and recipient cornea and suture lines increased at 21 days after surgery and remained throughout the experimental period. The central portion of the transplanted cornea remained transparent while a corneal haze developed around the transplanted margin (Fig. 2). Stromal edema of the donor cornea was present from immediately after surgery until three weeks after surgery in all three beagles (Fig. 3), then began to decrease from four weeks after surgery. The corneal thickness was significantly increased at the day 7, 14, 21, and 28 after surgery compared to the CCT of pre-operative examined values (Table 1). The menace response was normal even though the transplanted corneas were edematous.


Deep anterior lamellar keratoplasty of dog eyes using the big-bubble technique
Slit-lamp biomicroscopy with direct diffuse illumination of the corneas under 10× magnification. (A) 12 h after surgery, (B) 7 days, (C) 14 days, (D) 21 days, (E) 28 days and (F) 150 days after surgery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037302&req=5

Figure 2: Slit-lamp biomicroscopy with direct diffuse illumination of the corneas under 10× magnification. (A) 12 h after surgery, (B) 7 days, (C) 14 days, (D) 21 days, (E) 28 days and (F) 150 days after surgery.
Mentions: A corneal haze was developed from 7 days after surgery at the central transplanted cornea and around the suture line (Table 1). The corneal haze of central corneal part was reduced from 21 days after surgery. However, the corneal haze around the junction of the donor and recipient cornea and suture lines increased at 21 days after surgery and remained throughout the experimental period. The central portion of the transplanted cornea remained transparent while a corneal haze developed around the transplanted margin (Fig. 2). Stromal edema of the donor cornea was present from immediately after surgery until three weeks after surgery in all three beagles (Fig. 3), then began to decrease from four weeks after surgery. The corneal thickness was significantly increased at the day 7, 14, 21, and 28 after surgery compared to the CCT of pre-operative examined values (Table 1). The menace response was normal even though the transplanted corneas were edematous.

View Article: PubMed Central - PubMed

ABSTRACT

This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 µm, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet's membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7 days and 2% cyclosporine ointment every 12 h for 1 month. The eyes were examined post-operatively at 7, 14, 21, 28 and 150 days. The central portion of the transplanted cornea stayed transparent while corneal haze developed around the transplanted margin. Menace response was normal even though the transplanted cornea was edematous until 3 weeks after surgery. A marginal haze was rarely observed between the donor and recipient corneas at 150 days after the operation. A spotted haze developed in the central part of the deep stroma near the DM. Upon histopathological examination, the stroma and epithelium of the donor cornea had normal structures. Corneal transplantation using DALK with BBT can be performed in dogs preserving the healthy endothelium.

No MeSH data available.


Related in: MedlinePlus