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Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera

View Article: PubMed Central - PubMed

ABSTRACT

Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are important for preventing SFTS. Monoclonal antibodies against the recombinant nucleoprotein of SFTSV were generated to develop a competitive enzyme-linked immunosorbent assay (cELISA) for the detection of antibodies against SFTSV infection in cattle. The specificity and sensitivity of cELISA was assessed by comparing the results of this assay to those of an immunofluorescence assay (IFA). The results of the cELISA using 416 field bovine serum samples and laboratory-immunized positive sera showed 98.1% consistency with those of the IFA. The cELISA used in this study did not show cross-reactivity with antisera against other viral cattle diseases. The cELISA presented in this study can be applied to detect antibodies against SFTSV in cattle.

No MeSH data available.


Antibody responses from laboratory immunized cattle. The SFTSV-positive bovine serum was tested in the IFA. The optimized dilution rate of the positive serum samples was 1/80, while less diluted sera (1/10 and 1/50) showed strong background signals in the IFA.
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Figure 3: Antibody responses from laboratory immunized cattle. The SFTSV-positive bovine serum was tested in the IFA. The optimized dilution rate of the positive serum samples was 1/80, while less diluted sera (1/10 and 1/50) showed strong background signals in the IFA.

Mentions: SFTSV-positive cattle sera were generated from two cattle that were injected three times with inactivated SFTSV. Serially diluted positive or negative serum samples were tested to determine the optimized dilution rate to distinguish SFTSV-positive serum samples from negative samples in IFA. The maximum dilution rate of serum for the IFA at which a non-specific background signal was not observed in the negative control was 1/80 (Fig. 3).


Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
Antibody responses from laboratory immunized cattle. The SFTSV-positive bovine serum was tested in the IFA. The optimized dilution rate of the positive serum samples was 1/80, while less diluted sera (1/10 and 1/50) showed strong background signals in the IFA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037297&req=5

Figure 3: Antibody responses from laboratory immunized cattle. The SFTSV-positive bovine serum was tested in the IFA. The optimized dilution rate of the positive serum samples was 1/80, while less diluted sera (1/10 and 1/50) showed strong background signals in the IFA.
Mentions: SFTSV-positive cattle sera were generated from two cattle that were injected three times with inactivated SFTSV. Serially diluted positive or negative serum samples were tested to determine the optimized dilution rate to distinguish SFTSV-positive serum samples from negative samples in IFA. The maximum dilution rate of serum for the IFA at which a non-specific background signal was not observed in the negative control was 1/80 (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are important for preventing SFTS. Monoclonal antibodies against the recombinant nucleoprotein of SFTSV were generated to develop a competitive enzyme-linked immunosorbent assay (cELISA) for the detection of antibodies against SFTSV infection in cattle. The specificity and sensitivity of cELISA was assessed by comparing the results of this assay to those of an immunofluorescence assay (IFA). The results of the cELISA using 416 field bovine serum samples and laboratory-immunized positive sera showed 98.1% consistency with those of the IFA. The cELISA used in this study did not show cross-reactivity with antisera against other viral cattle diseases. The cELISA presented in this study can be applied to detect antibodies against SFTSV in cattle.

No MeSH data available.