Limits...
Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood

View Article: PubMed Central - PubMed

ABSTRACT

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

No MeSH data available.


Related in: MedlinePlus

Proliferation of allogeneic lymphocytes when co-cultured with UCB-derived MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in vitro (n = 5). The Con A-activated allogeneic lymphocytes were cultured with or without MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in a RPMI-1640 medium with 10% FBS, L-glutamine and antibiotics in a CO2 incubator at 37℃ for 3 days. There was no significant (p > 0.05) inhibition of the proliferation of lymphocytes when they were co-cultured with MSC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037295&req=5

Figure 6: Proliferation of allogeneic lymphocytes when co-cultured with UCB-derived MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in vitro (n = 5). The Con A-activated allogeneic lymphocytes were cultured with or without MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in a RPMI-1640 medium with 10% FBS, L-glutamine and antibiotics in a CO2 incubator at 37℃ for 3 days. There was no significant (p > 0.05) inhibition of the proliferation of lymphocytes when they were co-cultured with MSC.

Mentions: To determine if MSC could affect the proliferation of allogeneic lymphocytes, MSC were co-cultured with Con A activated allogeneic lymphocytes at ratios of 1 : 1 to 1 : 5 in vitro for three days. As shown in Fig. 6, at a MSC/lymphocyte ratio of 1 : 1 and 1 : 5, the proliferation ratio of activated lymphocytes (47.20 ± 4.21% and 35.60 ± 5.80) was not significantly (p > 0.05) decreased relative to the lymphocytes only group (52.20 ± 7.88).


Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood
Proliferation of allogeneic lymphocytes when co-cultured with UCB-derived MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in vitro (n = 5). The Con A-activated allogeneic lymphocytes were cultured with or without MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in a RPMI-1640 medium with 10% FBS, L-glutamine and antibiotics in a CO2 incubator at 37℃ for 3 days. There was no significant (p > 0.05) inhibition of the proliferation of lymphocytes when they were co-cultured with MSC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037295&req=5

Figure 6: Proliferation of allogeneic lymphocytes when co-cultured with UCB-derived MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in vitro (n = 5). The Con A-activated allogeneic lymphocytes were cultured with or without MSC at ratios of 0 : 1, 1 : 1 and 1 : 5 in a RPMI-1640 medium with 10% FBS, L-glutamine and antibiotics in a CO2 incubator at 37℃ for 3 days. There was no significant (p > 0.05) inhibition of the proliferation of lymphocytes when they were co-cultured with MSC.
Mentions: To determine if MSC could affect the proliferation of allogeneic lymphocytes, MSC were co-cultured with Con A activated allogeneic lymphocytes at ratios of 1 : 1 to 1 : 5 in vitro for three days. As shown in Fig. 6, at a MSC/lymphocyte ratio of 1 : 1 and 1 : 5, the proliferation ratio of activated lymphocytes (47.20 ± 4.21% and 35.60 ± 5.80) was not significantly (p > 0.05) decreased relative to the lymphocytes only group (52.20 ± 7.88).

View Article: PubMed Central - PubMed

ABSTRACT

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

No MeSH data available.


Related in: MedlinePlus