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Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood

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ABSTRACT

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

No MeSH data available.


Effect of MSC on apoptosis of allogeneic lymphocytes following co-culture at ratios of 1 : 1 and 1 : 5 for 3 days. The test was conducted by Annexin-V and PI double staining and analyzed by flow cytometry. Apoptosis of MSC gated on size (A–C) and apoptosis of lymphocytes gated on size (D–F) were analyzed following MSC and activated lymphocytes were cultured alone (A and D) or cocultured at a ratio of 1 : 1 (B and E) and 1 : 5 (C and F).
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Figure 5: Effect of MSC on apoptosis of allogeneic lymphocytes following co-culture at ratios of 1 : 1 and 1 : 5 for 3 days. The test was conducted by Annexin-V and PI double staining and analyzed by flow cytometry. Apoptosis of MSC gated on size (A–C) and apoptosis of lymphocytes gated on size (D–F) were analyzed following MSC and activated lymphocytes were cultured alone (A and D) or cocultured at a ratio of 1 : 1 (B and E) and 1 : 5 (C and F).

Mentions: To determine if the proliferation of MSC was affected by allogeneic lymphocytes, MSC were co-cultured with Con A-activated allogeneic lymphocytes at a ratio of 1 : 1 to 1 : 5 in vitro for three days. As shown in Fig. 4, the proliferation rate of MSC at a ratio of 1 : 1 to 1 : 5 was increased to 134.6 ± 18.9% and 120.0 ± 23.2% relative to the control (104.0 ± 5.1%), respectively. The MSC did not show any significant interference with proliferation by the allogeneic Con A-activated lymphocytes (Fig. 4), and apoptosis analysis by flow cytometry did not show any induction of apoptosis in MSC by allogeneic lymphocytes (Fig. 5).


Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood
Effect of MSC on apoptosis of allogeneic lymphocytes following co-culture at ratios of 1 : 1 and 1 : 5 for 3 days. The test was conducted by Annexin-V and PI double staining and analyzed by flow cytometry. Apoptosis of MSC gated on size (A–C) and apoptosis of lymphocytes gated on size (D–F) were analyzed following MSC and activated lymphocytes were cultured alone (A and D) or cocultured at a ratio of 1 : 1 (B and E) and 1 : 5 (C and F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037295&req=5

Figure 5: Effect of MSC on apoptosis of allogeneic lymphocytes following co-culture at ratios of 1 : 1 and 1 : 5 for 3 days. The test was conducted by Annexin-V and PI double staining and analyzed by flow cytometry. Apoptosis of MSC gated on size (A–C) and apoptosis of lymphocytes gated on size (D–F) were analyzed following MSC and activated lymphocytes were cultured alone (A and D) or cocultured at a ratio of 1 : 1 (B and E) and 1 : 5 (C and F).
Mentions: To determine if the proliferation of MSC was affected by allogeneic lymphocytes, MSC were co-cultured with Con A-activated allogeneic lymphocytes at a ratio of 1 : 1 to 1 : 5 in vitro for three days. As shown in Fig. 4, the proliferation rate of MSC at a ratio of 1 : 1 to 1 : 5 was increased to 134.6 ± 18.9% and 120.0 ± 23.2% relative to the control (104.0 ± 5.1%), respectively. The MSC did not show any significant interference with proliferation by the allogeneic Con A-activated lymphocytes (Fig. 4), and apoptosis analysis by flow cytometry did not show any induction of apoptosis in MSC by allogeneic lymphocytes (Fig. 5).

View Article: PubMed Central - PubMed

ABSTRACT

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

No MeSH data available.