Limits...
Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood

View Article: PubMed Central - PubMed

ABSTRACT

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

No MeSH data available.


Expression of human HLA-ABC on the surface of human undifferentiated (A–C) and differentiated (D–F) MSC by immunocytochemical stain (green color). The nuclei of the MSC were counterstained with DAPI (blue color). (A and D) DAPI stain. (B and E) FITC stain. (C and F) Merged. 200× (A–C), 40× (D–F). Sale bars = 20 µm (A–C), 100 µm (D–F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037295&req=5

Figure 2: Expression of human HLA-ABC on the surface of human undifferentiated (A–C) and differentiated (D–F) MSC by immunocytochemical stain (green color). The nuclei of the MSC were counterstained with DAPI (blue color). (A and D) DAPI stain. (B and E) FITC stain. (C and F) Merged. 200× (A–C), 40× (D–F). Sale bars = 20 µm (A–C), 100 µm (D–F).

Mentions: When the immunophenotypes of undifferentiated and differentiated MSC were determined by immunocytochemistry, the undifferentiated MSC (panels A-C in Fig. 2) showed moderate expression of HLA-ABC antigen compared to chondrocytes (panels D-F in Fig. 2). Evaluation of the expression of HLA-DR antigen revealed negative expression by MSC (panels A-C in Fig. 3) and chondrocytes (panels D-F in Fig. 3).


Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood
Expression of human HLA-ABC on the surface of human undifferentiated (A–C) and differentiated (D–F) MSC by immunocytochemical stain (green color). The nuclei of the MSC were counterstained with DAPI (blue color). (A and D) DAPI stain. (B and E) FITC stain. (C and F) Merged. 200× (A–C), 40× (D–F). Sale bars = 20 µm (A–C), 100 µm (D–F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037295&req=5

Figure 2: Expression of human HLA-ABC on the surface of human undifferentiated (A–C) and differentiated (D–F) MSC by immunocytochemical stain (green color). The nuclei of the MSC were counterstained with DAPI (blue color). (A and D) DAPI stain. (B and E) FITC stain. (C and F) Merged. 200× (A–C), 40× (D–F). Sale bars = 20 µm (A–C), 100 µm (D–F).
Mentions: When the immunophenotypes of undifferentiated and differentiated MSC were determined by immunocytochemistry, the undifferentiated MSC (panels A-C in Fig. 2) showed moderate expression of HLA-ABC antigen compared to chondrocytes (panels D-F in Fig. 2). Evaluation of the expression of HLA-DR antigen revealed negative expression by MSC (panels A-C in Fig. 3) and chondrocytes (panels D-F in Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.

No MeSH data available.