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Hydration status affects osteopontin expression in the rat kidney

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ABSTRACT

Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration induced a significant increase in OPN protein expression, whereas increased fluid intake induced a decrease in protein expression. Under control conditions, OPN protein and mRNA expression were only detected in the descending thin limb (DTL). Dehydration induced increased expression in the DTL and the development of detectable expression in the thick ascending limb (TAL). In contrast, OPN expression levels declined to less than the controls in the DTL after hydration, while no expression of either protein or mRNA was detectable in the TAL. Immunoelectron microscopy demonstrated that hydration status altered tubular ultrastructure and intracellular OPN expression in the Golgi apparatus and secretory cytoplasmic vesicles. These data confirm that changes in oral fluid intake can regulate renal tubular epithelial cell OPN expression.

No MeSH data available.


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Transmission electron micrographs illustrating OPN immunostaining in the thick ascending limb (asterisk) from dehydrated (A) and hydrated (B) rats. OPN immunoreactivity is observed in the subapical secretory vesicles in dehydrated animals. Note a rough surface apical membrane in hydrated animals. Scale bar = 5 µm.
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Figure 5: Transmission electron micrographs illustrating OPN immunostaining in the thick ascending limb (asterisk) from dehydrated (A) and hydrated (B) rats. OPN immunoreactivity is observed in the subapical secretory vesicles in dehydrated animals. Note a rough surface apical membrane in hydrated animals. Scale bar = 5 µm.

Mentions: In dehydrated animals, strong OPN immunostaining was also observed in the Golgi apparatus and numerous small vesicles in thick ascending limb cells (panel A in Fig. 5). OPN expression was not detected in the thick ascending limb in hydrated animals (panel B in Fig. 5). In general, the surface of the thick ascending limb was smooth in dehydrated animals, while it was rough in hydrated animals (Fig. 5).


Hydration status affects osteopontin expression in the rat kidney
Transmission electron micrographs illustrating OPN immunostaining in the thick ascending limb (asterisk) from dehydrated (A) and hydrated (B) rats. OPN immunoreactivity is observed in the subapical secretory vesicles in dehydrated animals. Note a rough surface apical membrane in hydrated animals. Scale bar = 5 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037293&req=5

Figure 5: Transmission electron micrographs illustrating OPN immunostaining in the thick ascending limb (asterisk) from dehydrated (A) and hydrated (B) rats. OPN immunoreactivity is observed in the subapical secretory vesicles in dehydrated animals. Note a rough surface apical membrane in hydrated animals. Scale bar = 5 µm.
Mentions: In dehydrated animals, strong OPN immunostaining was also observed in the Golgi apparatus and numerous small vesicles in thick ascending limb cells (panel A in Fig. 5). OPN expression was not detected in the thick ascending limb in hydrated animals (panel B in Fig. 5). In general, the surface of the thick ascending limb was smooth in dehydrated animals, while it was rough in hydrated animals (Fig. 5).

View Article: PubMed Central - PubMed

ABSTRACT

Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration induced a significant increase in OPN protein expression, whereas increased fluid intake induced a decrease in protein expression. Under control conditions, OPN protein and mRNA expression were only detected in the descending thin limb (DTL). Dehydration induced increased expression in the DTL and the development of detectable expression in the thick ascending limb (TAL). In contrast, OPN expression levels declined to less than the controls in the DTL after hydration, while no expression of either protein or mRNA was detectable in the TAL. Immunoelectron microscopy demonstrated that hydration status altered tubular ultrastructure and intracellular OPN expression in the Golgi apparatus and secretory cytoplasmic vesicles. These data confirm that changes in oral fluid intake can regulate renal tubular epithelial cell OPN expression.

No MeSH data available.


Related in: MedlinePlus