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Transgenesis for pig models

View Article: PubMed Central - PubMed

ABSTRACT

Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate specific gene-deleted and -inserted pig models. In the future, the development of pig models with gene editing technologies could be a valuable resource for biomedical research.

No MeSH data available.


Conditional gene expression with or without doxycycline. (A) Illustration of Tet-on gene expression by doxycycline. (B) RFP expression (left; with doxycycline) and non-expression (right; without doxycyline) in porcine fibroblasts after transfection of tet-on RFP vector.
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Figure 4: Conditional gene expression with or without doxycycline. (A) Illustration of Tet-on gene expression by doxycycline. (B) RFP expression (left; with doxycycline) and non-expression (right; without doxycyline) in porcine fibroblasts after transfection of tet-on RFP vector.

Mentions: Tet-on/off. The Tet-on/off system is a powerful tool for understanding the relationships involved in gene expression. Basically, the Tet-on/off system regulates the expression of genes by tetracycline (i.e., doxycycline) indicating that the target gene expression can be on or off at specific time. Consequently, this system can be a useful model for understanding both time-dependent gene expression and specific gene expression (Fig. 4). Various studies of mice using tet-on/off models have been conducted. In addition, two pig studies for producing live transgenic piglets using this system have been carried out [1314].


Transgenesis for pig models
Conditional gene expression with or without doxycycline. (A) Illustration of Tet-on gene expression by doxycycline. (B) RFP expression (left; with doxycycline) and non-expression (right; without doxycyline) in porcine fibroblasts after transfection of tet-on RFP vector.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037292&req=5

Figure 4: Conditional gene expression with or without doxycycline. (A) Illustration of Tet-on gene expression by doxycycline. (B) RFP expression (left; with doxycycline) and non-expression (right; without doxycyline) in porcine fibroblasts after transfection of tet-on RFP vector.
Mentions: Tet-on/off. The Tet-on/off system is a powerful tool for understanding the relationships involved in gene expression. Basically, the Tet-on/off system regulates the expression of genes by tetracycline (i.e., doxycycline) indicating that the target gene expression can be on or off at specific time. Consequently, this system can be a useful model for understanding both time-dependent gene expression and specific gene expression (Fig. 4). Various studies of mice using tet-on/off models have been conducted. In addition, two pig studies for producing live transgenic piglets using this system have been carried out [1314].

View Article: PubMed Central - PubMed

ABSTRACT

Animal models, particularly pigs, have come to play an important role in translational biomedical research. There have been many pig models with genetically modifications via somatic cell nuclear transfer (SCNT). However, because most transgenic pigs have been produced by random integration to date, the necessity for more exact gene-mutated models using recombinase based conditional gene expression like mice has been raised. Currently, advanced genome-editing technologies enable us to generate specific gene-deleted and -inserted pig models. In the future, the development of pig models with gene editing technologies could be a valuable resource for biomedical research.

No MeSH data available.