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Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes

View Article: PubMed Central - PubMed

ABSTRACT

Microbial populations living in environments with temperatures above 50°C (thermophiles) have been widely studied, increasing our knowledge in the composition and function of these ecological communities. Since these populations express a broad number of heat-resistant enzymes (thermozymes), they also represent an important source for novel biocatalysts that can be potentially used in industrial processes. The integrated study of the whole-community DNA from an environment, known as metagenomics, coupled with the development of next generation sequencing (NGS) technologies, has allowed the generation of large amounts of data from thermophiles. In this review, we summarize the main approaches commonly utilized for assessing the taxonomic and functional diversity of thermophiles through metagenomics, including several bioinformatics tools and some metagenome-derived methods to isolate their thermozymes.

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The two major strategies used for screening metagenomes in search of new thermozymes.
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Figure 2: The two major strategies used for screening metagenomes in search of new thermozymes.

Mentions: Function-based metagenomics relies on the construction of metagenomic libraries by cloning environmental DNA into expression vectors and propagating them in the appropriate hosts, followed by activity-based screening. After an active clone is identified, the sequence of the clone is determined, the gene of interest is amplified and cloned with the subsequent expression and characterization of the product to explore its biotechnological potential (Figure 2). This technique has the advantage of not requiring the cultivation of the native microorganisms or previous sequence information of known genes, thus representing a valuable approach for mining enzymes with new features.


Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes
The two major strategies used for screening metagenomes in search of new thermozymes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037290&req=5

Figure 2: The two major strategies used for screening metagenomes in search of new thermozymes.
Mentions: Function-based metagenomics relies on the construction of metagenomic libraries by cloning environmental DNA into expression vectors and propagating them in the appropriate hosts, followed by activity-based screening. After an active clone is identified, the sequence of the clone is determined, the gene of interest is amplified and cloned with the subsequent expression and characterization of the product to explore its biotechnological potential (Figure 2). This technique has the advantage of not requiring the cultivation of the native microorganisms or previous sequence information of known genes, thus representing a valuable approach for mining enzymes with new features.

View Article: PubMed Central - PubMed

ABSTRACT

Microbial populations living in environments with temperatures above 50°C (thermophiles) have been widely studied, increasing our knowledge in the composition and function of these ecological communities. Since these populations express a broad number of heat-resistant enzymes (thermozymes), they also represent an important source for novel biocatalysts that can be potentially used in industrial processes. The integrated study of the whole-community DNA from an environment, known as metagenomics, coupled with the development of next generation sequencing (NGS) technologies, has allowed the generation of large amounts of data from thermophiles. In this review, we summarize the main approaches commonly utilized for assessing the taxonomic and functional diversity of thermophiles through metagenomics, including several bioinformatics tools and some metagenome-derived methods to isolate their thermozymes.

No MeSH data available.