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HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum

View Article: PubMed Central - PubMed

ABSTRACT

Radial glial cells (RGs) are one of the important progenitor cells that can differentiate into neurons or glia to form functional neural circuits in the developing central nervous system (CNS). Histone deacetylases (HDACs) has been associated with visual activity dependent changes in BrdU-positive progenitor cells in the developing brain. We previously have shown that HDAC1 is involved in the experience-dependent proliferation of RGs. However, it is less clear whether two other members of class I HDACs, HDAC2 and HDAC3, are involved in the regulation of radial glia proliferation. Here, we reported that HDAC2 and HDAC3 expression were developmentally regulated in tectal cells, especially in the ventricular layer of the BLBP-positive RGs. Pharmacological blockade using an inhibitor of class I HDACs, MS-275, decreased the number of BrdU-positive dividing progenitor cells. Specific knockdown of HDAC3 but not HDAC2 decreased the number of BrdU- and BLBP-labeled cells, suggesting that the proliferation of radial glia was selectively mediated by HDAC3. Visual deprivation induced selective augmentation of histone H4 acetylation at lysine 16 in BLBP-positive cells. Furthermore, the visual deprivation-induced increase in BrdU-positive cells was partially blocked by HDAC3 downregulation but not by HDAC2 knockdown at stage 49 tadpoles. These data revealed a specific role of HDAC3 in experience-dependent radial glia proliferation during the development of Xenopus tectum.

No MeSH data available.


Visual experience induces selective increase of histone H4 acetylation at lysine 16. (A) Fluorescent images showing representative H4K16Ac+ cells in control (A1–A4), and VD (A5–A8) tadpoles. Scale: 50 μm. (B) Summary data revealed that visual deprivation increased the number of H4K16+ cells with enhanced fluorescence, which are colocalized with BLBP-labeled cells along the midline. (C) Summary data showing that VD barely changed the number of H2BK5+ cells with enhanced fluorescence. **p < 0.01.
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Figure 6: Visual experience induces selective increase of histone H4 acetylation at lysine 16. (A) Fluorescent images showing representative H4K16Ac+ cells in control (A1–A4), and VD (A5–A8) tadpoles. Scale: 50 μm. (B) Summary data revealed that visual deprivation increased the number of H4K16+ cells with enhanced fluorescence, which are colocalized with BLBP-labeled cells along the midline. (C) Summary data showing that VD barely changed the number of H2BK5+ cells with enhanced fluorescence. **p < 0.01.

Mentions: To determine whether the histone acetylation was involved in the VD-induced increase of RG proliferation, tadpoles were exposed to VD and immunofluorescence was performed using the following antibodies: H2BK5Ac, H4K5Ac, H4K8Ac, H4K12Ac, H4K16Ac and H3K9Ac. Interestingly, VD stimulation significantly increased the number of enhanced fluorescent cells with acetylated H4K16 along the tectal midline, which are BLBP+ RGs (Figures 6A,B). The acetylation levels of H2BK5Ac (Figure 6C), H4K5Ac, H4K8Ac, H4K12Ac and H3K9Ac (data not shown) were not significantly changed following VD stimulation. These results indicated that VD stimulation induced a selective increase in the number of H4K16Ac+ RGs in Xenopus tadpoles.


HDAC3 But not HDAC2 Mediates Visual Experience-Dependent Radial Glia Proliferation in the Developing Xenopus Tectum
Visual experience induces selective increase of histone H4 acetylation at lysine 16. (A) Fluorescent images showing representative H4K16Ac+ cells in control (A1–A4), and VD (A5–A8) tadpoles. Scale: 50 μm. (B) Summary data revealed that visual deprivation increased the number of H4K16+ cells with enhanced fluorescence, which are colocalized with BLBP-labeled cells along the midline. (C) Summary data showing that VD barely changed the number of H2BK5+ cells with enhanced fluorescence. **p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037170&req=5

Figure 6: Visual experience induces selective increase of histone H4 acetylation at lysine 16. (A) Fluorescent images showing representative H4K16Ac+ cells in control (A1–A4), and VD (A5–A8) tadpoles. Scale: 50 μm. (B) Summary data revealed that visual deprivation increased the number of H4K16+ cells with enhanced fluorescence, which are colocalized with BLBP-labeled cells along the midline. (C) Summary data showing that VD barely changed the number of H2BK5+ cells with enhanced fluorescence. **p < 0.01.
Mentions: To determine whether the histone acetylation was involved in the VD-induced increase of RG proliferation, tadpoles were exposed to VD and immunofluorescence was performed using the following antibodies: H2BK5Ac, H4K5Ac, H4K8Ac, H4K12Ac, H4K16Ac and H3K9Ac. Interestingly, VD stimulation significantly increased the number of enhanced fluorescent cells with acetylated H4K16 along the tectal midline, which are BLBP+ RGs (Figures 6A,B). The acetylation levels of H2BK5Ac (Figure 6C), H4K5Ac, H4K8Ac, H4K12Ac and H3K9Ac (data not shown) were not significantly changed following VD stimulation. These results indicated that VD stimulation induced a selective increase in the number of H4K16Ac+ RGs in Xenopus tadpoles.

View Article: PubMed Central - PubMed

ABSTRACT

Radial glial cells (RGs) are one of the important progenitor cells that can differentiate into neurons or glia to form functional neural circuits in the developing central nervous system (CNS). Histone deacetylases (HDACs) has been associated with visual activity dependent changes in BrdU-positive progenitor cells in the developing brain. We previously have shown that HDAC1 is involved in the experience-dependent proliferation of RGs. However, it is less clear whether two other members of class I HDACs, HDAC2 and HDAC3, are involved in the regulation of radial glia proliferation. Here, we reported that HDAC2 and HDAC3 expression were developmentally regulated in tectal cells, especially in the ventricular layer of the BLBP-positive RGs. Pharmacological blockade using an inhibitor of class I HDACs, MS-275, decreased the number of BrdU-positive dividing progenitor cells. Specific knockdown of HDAC3 but not HDAC2 decreased the number of BrdU- and BLBP-labeled cells, suggesting that the proliferation of radial glia was selectively mediated by HDAC3. Visual deprivation induced selective augmentation of histone H4 acetylation at lysine 16 in BLBP-positive cells. Furthermore, the visual deprivation-induced increase in BrdU-positive cells was partially blocked by HDAC3 downregulation but not by HDAC2 knockdown at stage 49 tadpoles. These data revealed a specific role of HDAC3 in experience-dependent radial glia proliferation during the development of Xenopus tectum.

No MeSH data available.