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Electrically controlled variation of receptor affinity

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ABSTRACT

A concept of virtual sensor array based on electrically controlled variation of affinity properties of the receptor layer is described. It was realized on the base of integrated electrochemical chemotransistor containing polyaniline as the receptor layer. Electrical control of the redox state of polyaniline was performed in five-electrode configuration containing four electrodes for conductivity measurements and one Ag/AgCl reference electrode. All the electrodes were integrated on the same glass chip. A room-temperature ionic liquid was used for the electrical connection between the reference electrode and chemosensitive material. Conductivity measurements demonstrated effective potential-controlled electrochemical conversions of the receptor material between different redox states. Binding of trimethylamine at three different potentials, corresponding to the different states of the receptor material, was studied. Concentration dependencies and binding kinetics were analyzed. The results demonstrated that the kinetic as well as the equilibrium binding properties of the receptor layer can be controlled by electrical potential, thus providing a possibility to form a virtual sensor array using only a single sensing element.

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Concentration dependence of the sensor signal for different gate potentials. At high analyte concentrations, the statistical error is smaller than the size of the symbols. Inset the same dependences in double reciprocal coordinates
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Fig2: Concentration dependence of the sensor signal for different gate potentials. At high analyte concentrations, the statistical error is smaller than the size of the symbols. Inset the same dependences in double reciprocal coordinates

Mentions: Introduction of TMA into the measurement cell leads to the decrease of the sensor conductance (Fig. 1C). Depending on the concentration of TMA, the effect develops within seconds or minutes and leads to the constant conductance value. Sensor incubation in pure air results in the recovery of the initial sensor conductance within few minutes. Concentration dependence of the sensor signal is shown in Fig. 2. The concentration increase results in monotonous changes of the sensor signal. At high concentrations, this dependence reaches a saturation value which depends on the gate potential. This indicates that the properties of the sensor material at different potentials are different. Oxidation of PANI leads to a higher value of the sensor signal. The obtained concentration dependencies were fitted by Langmuir adsorption isotherms (continues curves in Fig. 2). The good fitting was confirmed by linearization in double reciprocal coordinates (Fig. 2, inset).Fig. 2


Electrically controlled variation of receptor affinity
Concentration dependence of the sensor signal for different gate potentials. At high analyte concentrations, the statistical error is smaller than the size of the symbols. Inset the same dependences in double reciprocal coordinates
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5037141&req=5

Fig2: Concentration dependence of the sensor signal for different gate potentials. At high analyte concentrations, the statistical error is smaller than the size of the symbols. Inset the same dependences in double reciprocal coordinates
Mentions: Introduction of TMA into the measurement cell leads to the decrease of the sensor conductance (Fig. 1C). Depending on the concentration of TMA, the effect develops within seconds or minutes and leads to the constant conductance value. Sensor incubation in pure air results in the recovery of the initial sensor conductance within few minutes. Concentration dependence of the sensor signal is shown in Fig. 2. The concentration increase results in monotonous changes of the sensor signal. At high concentrations, this dependence reaches a saturation value which depends on the gate potential. This indicates that the properties of the sensor material at different potentials are different. Oxidation of PANI leads to a higher value of the sensor signal. The obtained concentration dependencies were fitted by Langmuir adsorption isotherms (continues curves in Fig. 2). The good fitting was confirmed by linearization in double reciprocal coordinates (Fig. 2, inset).Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

A concept of virtual sensor array based on electrically controlled variation of affinity properties of the receptor layer is described. It was realized on the base of integrated electrochemical chemotransistor containing polyaniline as the receptor layer. Electrical control of the redox state of polyaniline was performed in five-electrode configuration containing four electrodes for conductivity measurements and one Ag/AgCl reference electrode. All the electrodes were integrated on the same glass chip. A room-temperature ionic liquid was used for the electrical connection between the reference electrode and chemosensitive material. Conductivity measurements demonstrated effective potential-controlled electrochemical conversions of the receptor material between different redox states. Binding of trimethylamine at three different potentials, corresponding to the different states of the receptor material, was studied. Concentration dependencies and binding kinetics were analyzed. The results demonstrated that the kinetic as well as the equilibrium binding properties of the receptor layer can be controlled by electrical potential, thus providing a possibility to form a virtual sensor array using only a single sensing element.

No MeSH data available.


Related in: MedlinePlus