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Application of Omics Technologies for Evaluation of Antibacterial Mechanisms of Action of Plant-Derived Products

View Article: PubMed Central - PubMed

ABSTRACT

In the face of increasing bacterial resistance to antibiotics currently in use, the search for new antimicrobial agents has received a boost in recent years, with natural products playing an important role in this field. In fact, several methods have been proposed to investigate the antibacterial activities of natural products. However, given that the ultimate aim is future therapeutic use as novel drugs, it is extremely necessary to elucidate their modes of action, stating the molecular effects in detail, and identifying their targets in the bacterial cell. This review analyzes the application of “omics technologies” to understand the antibacterial mechanisms of bioactive natural products, to stimulate research interest in this area and promote scientific collaborations. Some studies have been specifically highlighted herein by examining their procedures and results (targeted proteins and metabolic pathways). These approaches have the potential to provide new insights into our comprehension of antimicrobial resistance/susceptibility, creating new perspectives for the struggle against bacteria, and leading to the development of novel products in the future.

No MeSH data available.


Overview of applications of “omics technologies” to evaluate the mechanisms of action of natural antimicrobials (NAM). (A) Genomic approaches: the target can be discovered by comparing the DNA sequence of a NAM-resistant bacteria and normal bacteria. (B) Proteomic and transcriptomic approaches: target identification can be performed by evaluating differential expression of genes in strains treated or untreated with NAM. (C) Metabolomic analysis: the metabolic profile of a bacterium treated with NAM can be compared with the profile of different drugs with known actions, potentially leading to target identification.
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Figure 1: Overview of applications of “omics technologies” to evaluate the mechanisms of action of natural antimicrobials (NAM). (A) Genomic approaches: the target can be discovered by comparing the DNA sequence of a NAM-resistant bacteria and normal bacteria. (B) Proteomic and transcriptomic approaches: target identification can be performed by evaluating differential expression of genes in strains treated or untreated with NAM. (C) Metabolomic analysis: the metabolic profile of a bacterium treated with NAM can be compared with the profile of different drugs with known actions, potentially leading to target identification.

Mentions: Recent advances in “omics” technologies (genomics, transcriptomics, proteomics, and metabolomics) are attributed to innovative breakthroughs in genome sequencing, bioinformatics, and analytic tools such as liquid and gas chromatography and mass spectrometry, along with high-throughput technologies. Omics technologies have provided crucial insights into processes related to bacterial physiology, virulence, stress, and the MOA of antimicrobial compounds (Roemer and Boone, 2013; Tang, 2015; Figure 1). The use of these tools provides deeper and more robust data, and has greater potential to reveal new therapeutic targets than conventional assays. These new targets and their related pathways are critically important in the struggle to overcome drug resistance (Roemer and Boone, 2013). In this review, we will focus on the application of these methodologies to study the mechanisms of action of plant-derived antibacterial compounds.


Application of Omics Technologies for Evaluation of Antibacterial Mechanisms of Action of Plant-Derived Products
Overview of applications of “omics technologies” to evaluate the mechanisms of action of natural antimicrobials (NAM). (A) Genomic approaches: the target can be discovered by comparing the DNA sequence of a NAM-resistant bacteria and normal bacteria. (B) Proteomic and transcriptomic approaches: target identification can be performed by evaluating differential expression of genes in strains treated or untreated with NAM. (C) Metabolomic analysis: the metabolic profile of a bacterium treated with NAM can be compared with the profile of different drugs with known actions, potentially leading to target identification.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037136&req=5

Figure 1: Overview of applications of “omics technologies” to evaluate the mechanisms of action of natural antimicrobials (NAM). (A) Genomic approaches: the target can be discovered by comparing the DNA sequence of a NAM-resistant bacteria and normal bacteria. (B) Proteomic and transcriptomic approaches: target identification can be performed by evaluating differential expression of genes in strains treated or untreated with NAM. (C) Metabolomic analysis: the metabolic profile of a bacterium treated with NAM can be compared with the profile of different drugs with known actions, potentially leading to target identification.
Mentions: Recent advances in “omics” technologies (genomics, transcriptomics, proteomics, and metabolomics) are attributed to innovative breakthroughs in genome sequencing, bioinformatics, and analytic tools such as liquid and gas chromatography and mass spectrometry, along with high-throughput technologies. Omics technologies have provided crucial insights into processes related to bacterial physiology, virulence, stress, and the MOA of antimicrobial compounds (Roemer and Boone, 2013; Tang, 2015; Figure 1). The use of these tools provides deeper and more robust data, and has greater potential to reveal new therapeutic targets than conventional assays. These new targets and their related pathways are critically important in the struggle to overcome drug resistance (Roemer and Boone, 2013). In this review, we will focus on the application of these methodologies to study the mechanisms of action of plant-derived antibacterial compounds.

View Article: PubMed Central - PubMed

ABSTRACT

In the face of increasing bacterial resistance to antibiotics currently in use, the search for new antimicrobial agents has received a boost in recent years, with natural products playing an important role in this field. In fact, several methods have been proposed to investigate the antibacterial activities of natural products. However, given that the ultimate aim is future therapeutic use as novel drugs, it is extremely necessary to elucidate their modes of action, stating the molecular effects in detail, and identifying their targets in the bacterial cell. This review analyzes the application of “omics technologies” to understand the antibacterial mechanisms of bioactive natural products, to stimulate research interest in this area and promote scientific collaborations. Some studies have been specifically highlighted herein by examining their procedures and results (targeted proteins and metabolic pathways). These approaches have the potential to provide new insights into our comprehension of antimicrobial resistance/susceptibility, creating new perspectives for the struggle against bacteria, and leading to the development of novel products in the future.

No MeSH data available.