Limits...
Morbility, clinical data and proteomic analysis of IUGR and AGA newborns at different gestational ages

View Article: PubMed Central - PubMed

ABSTRACT

The data are related to the proteomic analysis of 43 newborns with intrauterine growth retardation (IUGR) and 45 newborns with appropriate weight for gestational age (AGA) carried out by separation via 2DE and analyzed by MS–TOF/TOF. All newborns were separated into three gestational age groups, "Very Preterm" 29–32 weeks, "Moderate Preterm" 33–36 weeks, and, "Term" ≥37weeks. From each newborn, blood was drawn three times from birth to 1 month life. High-abundant serum proteins were depleted, and the minority ones were separated by 2DE and analyzed for significant expression differences. The data reflect analytic and clinic variables analyzed globally and categorized by gestational age in relation to IUGR and the optimization of conditions for 2-DE separation. The data from this study are related to the research article entitled "Alterations of Protein Expression in Serum of Infants with Intrauterine Growth Restriction and Different Gestational Ages" (M.D. Ruis-González, M.D. Cañete, J.L. Gómez-Chaparro, N. Abril, R. Cañete, J. López-Barea, 2015) [1]. The present dataset of serum IUGR newborn proteome can be used as a reference for any study involving intrauterine growth restriction during the first month of life.

No MeSH data available.


Related in: MedlinePlus

Representative gels obtained for optimization of 2DE separation of high-abundance depleted human serum proteins via IEF with IPG Strips (11 cm) of pH 3–10 (A) or pH 4–7 (B).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037120&req=5

f0005: Representative gels obtained for optimization of 2DE separation of high-abundance depleted human serum proteins via IEF with IPG Strips (11 cm) of pH 3–10 (A) or pH 4–7 (B).

Mentions: For the development of 2-DE electrophoresis, we started carrying our 2-DE gels with a wide pH range (3–10), as shown in Fig. 1A. In these initial gels we observed that most of the proteins were in the pH range 4–7. Thus, we reasoned that the resolution would improve using IPG strips of narrower pH gradient. Thus, 2-DE electrophoresis was routinely carried out using IPG strips of 11 cm and pH range 4–7, as shown in Fig. 1B. Isoelectric focusing was performed using an Protean IEF Cell (BioRad) a 20 °C according the next program (Table 8).


Morbility, clinical data and proteomic analysis of IUGR and AGA newborns at different gestational ages
Representative gels obtained for optimization of 2DE separation of high-abundance depleted human serum proteins via IEF with IPG Strips (11 cm) of pH 3–10 (A) or pH 4–7 (B).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037120&req=5

f0005: Representative gels obtained for optimization of 2DE separation of high-abundance depleted human serum proteins via IEF with IPG Strips (11 cm) of pH 3–10 (A) or pH 4–7 (B).
Mentions: For the development of 2-DE electrophoresis, we started carrying our 2-DE gels with a wide pH range (3–10), as shown in Fig. 1A. In these initial gels we observed that most of the proteins were in the pH range 4–7. Thus, we reasoned that the resolution would improve using IPG strips of narrower pH gradient. Thus, 2-DE electrophoresis was routinely carried out using IPG strips of 11 cm and pH range 4–7, as shown in Fig. 1B. Isoelectric focusing was performed using an Protean IEF Cell (BioRad) a 20 °C according the next program (Table 8).

View Article: PubMed Central - PubMed

ABSTRACT

The data are related to the proteomic analysis of 43 newborns with intrauterine growth retardation (IUGR) and 45 newborns with appropriate weight for gestational age (AGA) carried out by separation via 2DE and analyzed by MS–TOF/TOF. All newborns were separated into three gestational age groups, "Very Preterm" 29–32 weeks, "Moderate Preterm" 33–36 weeks, and, "Term" ≥37weeks. From each newborn, blood was drawn three times from birth to 1 month life. High-abundant serum proteins were depleted, and the minority ones were separated by 2DE and analyzed for significant expression differences. The data reflect analytic and clinic variables analyzed globally and categorized by gestational age in relation to IUGR and the optimization of conditions for 2-DE separation. The data from this study are related to the research article entitled "Alterations of Protein Expression in Serum of Infants with Intrauterine Growth Restriction and Different Gestational Ages" (M.D. Ruis-González, M.D. Cañete, J.L. Gómez-Chaparro, N. Abril, R. Cañete, J. López-Barea, 2015) [1]. The present dataset of serum IUGR newborn proteome can be used as a reference for any study involving intrauterine growth restriction during the first month of life.

No MeSH data available.


Related in: MedlinePlus