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Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1

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ABSTRACT

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.

No MeSH data available.


Response surface plots of effect of KCl and Yeast extract on pectinase production
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Fig6: Response surface plots of effect of KCl and Yeast extract on pectinase production

Mentions: Similarly, KCl has shown significance on the pectinase production than yeast extract and MgSO4·7H2O, respectively (Figs. 5, 6). All the response surfaces could be analyzed for determining the optimized value of the factors. Enzyme production is a growing field of biotechnology, the majority of the industrial enzymes are of microbial origin. Though there is a conventional method for the optimization of media composition RSM is the best statistical method to optimise all media components at a time. Through this method we can find out the suitable medium with optimal cultural conditions for high production of microbial pectinase by submerged fermentation. In the present study, the Enterobacter sp. PSTB-1 showed pectinase activity ranging from 10.3852 U/ml (low) to 87.5565 U/ml (high) under RSM designed conditions. The highest activity 87.5565 U/ml of Enterobacter sp. PSTB-1 is higher than the pectinase activity 53 U/ml of Bacillus sp. DT7 and 10.65 U/ml of Bacillus licheniformis (Shahera et al. 2002; Hou et al. 2011).Fig. 5


Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1
Response surface plots of effect of KCl and Yeast extract on pectinase production
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037110&req=5

Fig6: Response surface plots of effect of KCl and Yeast extract on pectinase production
Mentions: Similarly, KCl has shown significance on the pectinase production than yeast extract and MgSO4·7H2O, respectively (Figs. 5, 6). All the response surfaces could be analyzed for determining the optimized value of the factors. Enzyme production is a growing field of biotechnology, the majority of the industrial enzymes are of microbial origin. Though there is a conventional method for the optimization of media composition RSM is the best statistical method to optimise all media components at a time. Through this method we can find out the suitable medium with optimal cultural conditions for high production of microbial pectinase by submerged fermentation. In the present study, the Enterobacter sp. PSTB-1 showed pectinase activity ranging from 10.3852 U/ml (low) to 87.5565 U/ml (high) under RSM designed conditions. The highest activity 87.5565 U/ml of Enterobacter sp. PSTB-1 is higher than the pectinase activity 53 U/ml of Bacillus sp. DT7 and 10.65 U/ml of Bacillus licheniformis (Shahera et al. 2002; Hou et al. 2011).Fig. 5

View Article: PubMed Central - PubMed

ABSTRACT

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.

No MeSH data available.