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Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1

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ABSTRACT

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.

No MeSH data available.


Response surface plots of effect of NaNO3 and KH2PO4 on pectinase production
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Fig3: Response surface plots of effect of NaNO3 and KH2PO4 on pectinase production

Mentions: The adjusted R2 was a corrected value for R2 after elimination of the unnecessary model terms. If many non-significant terms have been included in the model, the adjusted R2 would be remarkably smaller than the R2. The adjusted R2 was 0.3663, which is more suitable for comparing models with different numbers of independent variables. The coefficient of variation (CV) is a measure of residual variation of the data relative to the size of the mean; the small values of CV give better reproducibility. A lower value for the CV 36.30 % clearly indicates high degree of precision and higher reliability of the experimental values. The significance of individual variables can be evaluated from their p values, the more significant terms having a lower p-value. The p-values are used to check the significance of each coefficient which also indicates the interaction strength between each independent variable. The p-values of each of the variables also indicate their quadratic and interaction terms. Besides the relationship between the actual experimental and predicted values, the plotted points cluster around the diagonal line, indicating good fitness of the model and they are in reasonable agreement (Fig. 2). An effect of concentration of KH2PO4 has shown significance on the pectinase production than NaNO3 and MIW powder was analyzed by the response surface plots (Figs. 3, 4).Fig. 2


Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1
Response surface plots of effect of NaNO3 and KH2PO4 on pectinase production
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037110&req=5

Fig3: Response surface plots of effect of NaNO3 and KH2PO4 on pectinase production
Mentions: The adjusted R2 was a corrected value for R2 after elimination of the unnecessary model terms. If many non-significant terms have been included in the model, the adjusted R2 would be remarkably smaller than the R2. The adjusted R2 was 0.3663, which is more suitable for comparing models with different numbers of independent variables. The coefficient of variation (CV) is a measure of residual variation of the data relative to the size of the mean; the small values of CV give better reproducibility. A lower value for the CV 36.30 % clearly indicates high degree of precision and higher reliability of the experimental values. The significance of individual variables can be evaluated from their p values, the more significant terms having a lower p-value. The p-values are used to check the significance of each coefficient which also indicates the interaction strength between each independent variable. The p-values of each of the variables also indicate their quadratic and interaction terms. Besides the relationship between the actual experimental and predicted values, the plotted points cluster around the diagonal line, indicating good fitness of the model and they are in reasonable agreement (Fig. 2). An effect of concentration of KH2PO4 has shown significance on the pectinase production than NaNO3 and MIW powder was analyzed by the response surface plots (Figs. 3, 4).Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.

No MeSH data available.