Limits...
Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1

View Article: PubMed Central - PubMed

ABSTRACT

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.

No MeSH data available.


Phylogenetic analysis based on 16S r-RNA sequence available from bacterial isolates was constructed. Evolutionary distances were computed using the p-distance method clustering with the neighbour-joining method
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037110&req=5

Fig1: Phylogenetic analysis based on 16S r-RNA sequence available from bacterial isolates was constructed. Evolutionary distances were computed using the p-distance method clustering with the neighbour-joining method

Mentions: The 16S r-RNA sequence of isolated bacterial strain PSTB-1 has shown more homology with reported strain Enterobacter cloacae sub sp. Cloacae (Fig. 1). The identification of this strain was confirmed by NCBI sequence submission. Similarly, pectinolytic bacterial strains such as Bacillus firmus-I-4071, B. firmus-I-10104 and Bacillus laterosporus-I-107 were reported from agro and fruit processing industrial wastes (Geetha et al. 2012; Veeranjaneya Reddy et al. 2011; Kashyap et al. 2000).Fig. 1


Effects of the culture media optimization on pectinase production by Enterobacter sp. PSTB-1
Phylogenetic analysis based on 16S r-RNA sequence available from bacterial isolates was constructed. Evolutionary distances were computed using the p-distance method clustering with the neighbour-joining method
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037110&req=5

Fig1: Phylogenetic analysis based on 16S r-RNA sequence available from bacterial isolates was constructed. Evolutionary distances were computed using the p-distance method clustering with the neighbour-joining method
Mentions: The 16S r-RNA sequence of isolated bacterial strain PSTB-1 has shown more homology with reported strain Enterobacter cloacae sub sp. Cloacae (Fig. 1). The identification of this strain was confirmed by NCBI sequence submission. Similarly, pectinolytic bacterial strains such as Bacillus firmus-I-4071, B. firmus-I-10104 and Bacillus laterosporus-I-107 were reported from agro and fruit processing industrial wastes (Geetha et al. 2012; Veeranjaneya Reddy et al. 2011; Kashyap et al. 2000).Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2.0 g/l, KCl 0.50 g/l, KH2PO4 1.0 g/l, MgSO4·7H2O 0.50 g/l, Yeast extract 1.0 g/l, mango industrial waste powder 5.0 g/l. The actual pectinase activity was 75.23 % correlated with the predicted pectinase activity where the model (CCD) was significant. Response surface modelling applied effectively to optimize the production of pectinase in submerged fermentation to make the process low cost-effective by using powdered mango industrial waste as substrate.

No MeSH data available.