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The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus

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ABSTRACT

Aponogeton ulvaceus Baker (Aponogetonaceae) is a commercially important ornamental aquatic plant species with traditional medicinal uses. Due to the low survival rate of seedlings, propagation by conventional means has been met with many difficulties. In this study, botanical aspects of A. ulvaceus were examined with regards to the morphology, anatomy and physiology of the plant and an efficient protocol for its in vitro propagation using immature tuber explants has been established. The existence of glandular trichomes on the leaves was discovered and the occurrence of circumnutation in A. ulvaceus has been demonstrated. Immature tuber segments with meristems were cultured on MS medium supplemented with various combinations (0, 1, 2, and 3 mg/L) of BAP and NAA for callus induction. The highest percentage of callus production (100 %) was obtained in two different treatments: 1 mg/L BAP and 3 mg/L NAA, and 2 mg/L BAP and 3 mg/L NAA. For shoot and root organogenesis, the combination of 1 mg/L BAP and 1 mg/L NAA was shown to be significant for A. ulvaceus regeneration when compared to control, which yields a mean shoot and root number of 22.50 and 29.50 respectively. The current protocol is the first reported successful establishment of in vitro clonal propagation of A. ulvaceus.

Electronic supplementary material: The online version of this article (doi:10.1186/s40064-016-3041-4) contains supplementary material, which is available to authorized users.

No MeSH data available.


Top-view of the circumnutation trajectories of A. ulvaceus shoots. a Irregular zigzag circumnutation. Shoot movement marked by colour gradients beginning from red to blue line. Trajectory was recorded at 5 min intervals and extracted from CT. b Rosette-like circumnutation; scale bar 2 cm. Each point indicates tip position at 5 min intervals. Direction of movement as shown by arrowhead
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Fig3: Top-view of the circumnutation trajectories of A. ulvaceus shoots. a Irregular zigzag circumnutation. Shoot movement marked by colour gradients beginning from red to blue line. Trajectory was recorded at 5 min intervals and extracted from CT. b Rosette-like circumnutation; scale bar 2 cm. Each point indicates tip position at 5 min intervals. Direction of movement as shown by arrowhead

Mentions: The movements of a growing 4-day-old shoot were tracked using the time-lapse video method (Additional file 1: Video S1). Circumnutation parameters and trajectory of shoot apex were subsequently generated using CT software. The oscillation pattern of the shoot depicts a wide ellipse with a shape coefficient of 0.84 and it circumnutated in a counter-clockwise (ccw) direction (left-handed) (Fig. 3a). It was found that the tip organ assumes the irregularly zigzag trajectory of circumnutation, although previously it was demonstrated that rosette-like trajectory also occurs in A. ulvaceus (Fig. 3b). The circumnutation period of the shoot typically reaches 110 min with amplitude (length) of 77.15 mm. Taken together, these qualitative observations demonstrate the occurrence of circumnutation in the aquatic plant A. ulvaceus, especially in the actively growing parts of plant organs.Fig. 3


The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus
Top-view of the circumnutation trajectories of A. ulvaceus shoots. a Irregular zigzag circumnutation. Shoot movement marked by colour gradients beginning from red to blue line. Trajectory was recorded at 5 min intervals and extracted from CT. b Rosette-like circumnutation; scale bar 2 cm. Each point indicates tip position at 5 min intervals. Direction of movement as shown by arrowhead
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037101&req=5

Fig3: Top-view of the circumnutation trajectories of A. ulvaceus shoots. a Irregular zigzag circumnutation. Shoot movement marked by colour gradients beginning from red to blue line. Trajectory was recorded at 5 min intervals and extracted from CT. b Rosette-like circumnutation; scale bar 2 cm. Each point indicates tip position at 5 min intervals. Direction of movement as shown by arrowhead
Mentions: The movements of a growing 4-day-old shoot were tracked using the time-lapse video method (Additional file 1: Video S1). Circumnutation parameters and trajectory of shoot apex were subsequently generated using CT software. The oscillation pattern of the shoot depicts a wide ellipse with a shape coefficient of 0.84 and it circumnutated in a counter-clockwise (ccw) direction (left-handed) (Fig. 3a). It was found that the tip organ assumes the irregularly zigzag trajectory of circumnutation, although previously it was demonstrated that rosette-like trajectory also occurs in A. ulvaceus (Fig. 3b). The circumnutation period of the shoot typically reaches 110 min with amplitude (length) of 77.15 mm. Taken together, these qualitative observations demonstrate the occurrence of circumnutation in the aquatic plant A. ulvaceus, especially in the actively growing parts of plant organs.Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Aponogeton ulvaceus Baker (Aponogetonaceae) is a commercially important ornamental aquatic plant species with traditional medicinal uses. Due to the low survival rate of seedlings, propagation by conventional means has been met with many difficulties. In this study, botanical aspects of A. ulvaceus were examined with regards to the morphology, anatomy and physiology of the plant and an efficient protocol for its in vitro propagation using immature tuber explants has been established. The existence of glandular trichomes on the leaves was discovered and the occurrence of circumnutation in A. ulvaceus has been demonstrated. Immature tuber segments with meristems were cultured on MS medium supplemented with various combinations (0, 1, 2, and 3 mg/L) of BAP and NAA for callus induction. The highest percentage of callus production (100 %) was obtained in two different treatments: 1 mg/L BAP and 3 mg/L NAA, and 2 mg/L BAP and 3 mg/L NAA. For shoot and root organogenesis, the combination of 1 mg/L BAP and 1 mg/L NAA was shown to be significant for A. ulvaceus regeneration when compared to control, which yields a mean shoot and root number of 22.50 and 29.50 respectively. The current protocol is the first reported successful establishment of in vitro clonal propagation of A. ulvaceus.

Electronic supplementary material: The online version of this article (doi:10.1186/s40064-016-3041-4) contains supplementary material, which is available to authorized users.

No MeSH data available.