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Size effect of Au/PAMAM contrast agent on CT imaging of reticuloendothelial system and tumor tissue

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ABSTRACT

Polyamidoamine (PAMAM)-entrapped Au nanoparticles were synthesized with distinct sizes to figure out the size effect of Au-based contrast agent on CT imaging of passively targeted tissues. Au/PAMAM nanoparticles were first synthesized with narrow distribution of particles size of 22.2 ± 3.1, 54.2 ± 3.7, and 104.9 ± 4.7 nm in diameters. Size effect leads no significant difference on X-ray attenuation when Au/PAMAM was ≤0.05 mol/L. For CT imaging of a tumor model, small Au/PAMAM were more easily internalized via endocytosis in the liver, leading to more obviously enhanced contrast. Similarly, contrast agents with small sizes were more effective in tumor imaging because of the enhanced permeability and retention effect. Overall, the particle size of Au/PAMAM heavily affected the efficiency of CT enhancement in imaging RES and tumors.

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Size effect of Au-based contrast agent on CT imaging of subcutaneous A549 tumor. a–c CT imaging of tumor with 22.2, 54.2, and 104.9 nm Au/PAMAM injected. d Detailed CT values of tumor imaging. e HE staining of tumor tissues where no changes of cell morphology were observed. f Au content in corresponding tumors detected via ICP-AES at 4 h post injection
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Fig4: Size effect of Au-based contrast agent on CT imaging of subcutaneous A549 tumor. a–c CT imaging of tumor with 22.2, 54.2, and 104.9 nm Au/PAMAM injected. d Detailed CT values of tumor imaging. e HE staining of tumor tissues where no changes of cell morphology were observed. f Au content in corresponding tumors detected via ICP-AES at 4 h post injection

Mentions: For the enhanced CT imaging of tumors (Fig. 4a–c), tumorous tissues were more clearly outlined. In the quantitative analysis (Fig. 4d), CT values increased along with the accumulation of contrast agents, and size effect-induced differences on ΔHU were amplified due to the lasting accumulation of Au/PAMAM. No Au/PAMAM-induced changes of cell morphology was observed on HE staining (Fig. 4e), proving the biosafety in acute period after injection. As the fundamental reason of difference on CT enhancements, Au content in tumor tissues heavily relied on the particle size (Fig. 4f), but an obviously lower level of Au accumulation in the tumor was observed when compared with Au content in the liver, this is why the ΔHU for tumor imaging was smaller than the ΔHU in liver imaging.Fig. 4


Size effect of Au/PAMAM contrast agent on CT imaging of reticuloendothelial system and tumor tissue
Size effect of Au-based contrast agent on CT imaging of subcutaneous A549 tumor. a–c CT imaging of tumor with 22.2, 54.2, and 104.9 nm Au/PAMAM injected. d Detailed CT values of tumor imaging. e HE staining of tumor tissues where no changes of cell morphology were observed. f Au content in corresponding tumors detected via ICP-AES at 4 h post injection
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037097&req=5

Fig4: Size effect of Au-based contrast agent on CT imaging of subcutaneous A549 tumor. a–c CT imaging of tumor with 22.2, 54.2, and 104.9 nm Au/PAMAM injected. d Detailed CT values of tumor imaging. e HE staining of tumor tissues where no changes of cell morphology were observed. f Au content in corresponding tumors detected via ICP-AES at 4 h post injection
Mentions: For the enhanced CT imaging of tumors (Fig. 4a–c), tumorous tissues were more clearly outlined. In the quantitative analysis (Fig. 4d), CT values increased along with the accumulation of contrast agents, and size effect-induced differences on ΔHU were amplified due to the lasting accumulation of Au/PAMAM. No Au/PAMAM-induced changes of cell morphology was observed on HE staining (Fig. 4e), proving the biosafety in acute period after injection. As the fundamental reason of difference on CT enhancements, Au content in tumor tissues heavily relied on the particle size (Fig. 4f), but an obviously lower level of Au accumulation in the tumor was observed when compared with Au content in the liver, this is why the ΔHU for tumor imaging was smaller than the ΔHU in liver imaging.Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Polyamidoamine (PAMAM)-entrapped Au nanoparticles were synthesized with distinct sizes to figure out the size effect of Au-based contrast agent on CT imaging of passively targeted tissues. Au/PAMAM nanoparticles were first synthesized with narrow distribution of particles size of 22.2 ± 3.1, 54.2 ± 3.7, and 104.9 ± 4.7 nm in diameters. Size effect leads no significant difference on X-ray attenuation when Au/PAMAM was ≤0.05 mol/L. For CT imaging of a tumor model, small Au/PAMAM were more easily internalized via endocytosis in the liver, leading to more obviously enhanced contrast. Similarly, contrast agents with small sizes were more effective in tumor imaging because of the enhanced permeability and retention effect. Overall, the particle size of Au/PAMAM heavily affected the efficiency of CT enhancement in imaging RES and tumors.

No MeSH data available.


Related in: MedlinePlus