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Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ- db/db mice

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.

Materials/methods: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.

Results: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.

Conclusions: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

No MeSH data available.


Related in: MedlinePlus

Effect of G. procumbens extract (GPE) supplementation on G6Pase and PEPCK expression and hepatic glycogen in the liver of C57BL/KsJ-db/db mice.Representative blots of G6Pase and PEPCK protein expression are shown with protein expression levels quantified relative to the expression level observed in samples from db/db-control mice. Each value is expressed as mean ± SD of experiments performed in triplicate. a-c Values denoted by different letters are significantly different (P < 0.05), as analyzed by Duncan's multiple range test. PEPCK: Phosphoenolpyruvate carboxykinase, G6Pase: Glucose-6-phosphatase.
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Figure 4: Effect of G. procumbens extract (GPE) supplementation on G6Pase and PEPCK expression and hepatic glycogen in the liver of C57BL/KsJ-db/db mice.Representative blots of G6Pase and PEPCK protein expression are shown with protein expression levels quantified relative to the expression level observed in samples from db/db-control mice. Each value is expressed as mean ± SD of experiments performed in triplicate. a-c Values denoted by different letters are significantly different (P < 0.05), as analyzed by Duncan's multiple range test. PEPCK: Phosphoenolpyruvate carboxykinase, G6Pase: Glucose-6-phosphatase.

Mentions: As shown in Fig. 4, pAMPK activation in the liver of db/db-GPE mice was significantly higher than that in the db/db-control mice. The expression of G6Pase in the liver significantly decreased in db/db-GPE mice, with 0.5-fold lower levels than those observed in the db/db-control mice. Hepatic glycogen levels in the db/db-RG mice were the highest with values of 119.33 ± 4.04 mg/g of liver. db/db-GPE mice were significantly higher than the levels in the db/db-control mice, with values of 109.67 ± 4.16 and 90.25 ± 4.75 mg/g of liver measured in db/db-GPE and db/db-control mice, respectively (Fig. 4).


Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ- db/db mice
Effect of G. procumbens extract (GPE) supplementation on G6Pase and PEPCK expression and hepatic glycogen in the liver of C57BL/KsJ-db/db mice.Representative blots of G6Pase and PEPCK protein expression are shown with protein expression levels quantified relative to the expression level observed in samples from db/db-control mice. Each value is expressed as mean ± SD of experiments performed in triplicate. a-c Values denoted by different letters are significantly different (P < 0.05), as analyzed by Duncan's multiple range test. PEPCK: Phosphoenolpyruvate carboxykinase, G6Pase: Glucose-6-phosphatase.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037068&req=5

Figure 4: Effect of G. procumbens extract (GPE) supplementation on G6Pase and PEPCK expression and hepatic glycogen in the liver of C57BL/KsJ-db/db mice.Representative blots of G6Pase and PEPCK protein expression are shown with protein expression levels quantified relative to the expression level observed in samples from db/db-control mice. Each value is expressed as mean ± SD of experiments performed in triplicate. a-c Values denoted by different letters are significantly different (P < 0.05), as analyzed by Duncan's multiple range test. PEPCK: Phosphoenolpyruvate carboxykinase, G6Pase: Glucose-6-phosphatase.
Mentions: As shown in Fig. 4, pAMPK activation in the liver of db/db-GPE mice was significantly higher than that in the db/db-control mice. The expression of G6Pase in the liver significantly decreased in db/db-GPE mice, with 0.5-fold lower levels than those observed in the db/db-control mice. Hepatic glycogen levels in the db/db-RG mice were the highest with values of 119.33 ± 4.04 mg/g of liver. db/db-GPE mice were significantly higher than the levels in the db/db-control mice, with values of 109.67 ± 4.16 and 90.25 ± 4.75 mg/g of liver measured in db/db-GPE and db/db-control mice, respectively (Fig. 4).

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.

Materials/methods: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.

Results: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.

Conclusions: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

No MeSH data available.


Related in: MedlinePlus