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Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ- db/db mice

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.

Materials/methods: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.

Results: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.

Conclusions: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

No MeSH data available.


Related in: MedlinePlus

Effect of G. procumbens extract (GPE) supplementation on intraperitoneal glucose tolerance tests (A) and insulin tolerance tests (B) in C57BL/KsJ-db/db mice.Blood glucose concentrations were measured at the indicated times and presented as percentages of glucose measured at the time of injection (t = 0) of glucose (0.5 g/kg of BW) or insulin (2 units/kg of BW). db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group. a-c Mean values designated by different letters are significantly different between groups (P < 0.05).
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Figure 2: Effect of G. procumbens extract (GPE) supplementation on intraperitoneal glucose tolerance tests (A) and insulin tolerance tests (B) in C57BL/KsJ-db/db mice.Blood glucose concentrations were measured at the indicated times and presented as percentages of glucose measured at the time of injection (t = 0) of glucose (0.5 g/kg of BW) or insulin (2 units/kg of BW). db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group. a-c Mean values designated by different letters are significantly different between groups (P < 0.05).

Mentions: We performed IPGTT in db/db mice receiving GPE to evaluate GPE's ability to improve glucose tolerance. The results are presented as percentages of the measurement performed at the time of glucose injection (t = 0) and significant differences among db/db-control, db/db-RG and db/db-GPE group. As shown in Fig. 2 (A), blood glucose levels in the db/db-GPE mice peaked at 60 minutes after injection and recovered to levels close to the basal value at 120 min. A similar pattern was observed in mice from the db/db-RG group. IPITT was also performed to determine the effects of GPE supplementation on insulin tolerance. The results expressed as percentages of the measurement at the time of insulin injection (t = 0) and significant differences among db/db-control, db/db-RG, and db/db-GPE group are presented. As shown in Fig. 2 (B), db/db-GPE and db/db-RG mice exhibited a rapid reduction in blood glucose levels compared with the db/db-control mice, with blood glucose levels decreasing within 120 min of the insulin injection.


Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ- db/db mice
Effect of G. procumbens extract (GPE) supplementation on intraperitoneal glucose tolerance tests (A) and insulin tolerance tests (B) in C57BL/KsJ-db/db mice.Blood glucose concentrations were measured at the indicated times and presented as percentages of glucose measured at the time of injection (t = 0) of glucose (0.5 g/kg of BW) or insulin (2 units/kg of BW). db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group. a-c Mean values designated by different letters are significantly different between groups (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037068&req=5

Figure 2: Effect of G. procumbens extract (GPE) supplementation on intraperitoneal glucose tolerance tests (A) and insulin tolerance tests (B) in C57BL/KsJ-db/db mice.Blood glucose concentrations were measured at the indicated times and presented as percentages of glucose measured at the time of injection (t = 0) of glucose (0.5 g/kg of BW) or insulin (2 units/kg of BW). db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group. a-c Mean values designated by different letters are significantly different between groups (P < 0.05).
Mentions: We performed IPGTT in db/db mice receiving GPE to evaluate GPE's ability to improve glucose tolerance. The results are presented as percentages of the measurement performed at the time of glucose injection (t = 0) and significant differences among db/db-control, db/db-RG and db/db-GPE group. As shown in Fig. 2 (A), blood glucose levels in the db/db-GPE mice peaked at 60 minutes after injection and recovered to levels close to the basal value at 120 min. A similar pattern was observed in mice from the db/db-RG group. IPITT was also performed to determine the effects of GPE supplementation on insulin tolerance. The results expressed as percentages of the measurement at the time of insulin injection (t = 0) and significant differences among db/db-control, db/db-RG, and db/db-GPE group are presented. As shown in Fig. 2 (B), db/db-GPE and db/db-RG mice exhibited a rapid reduction in blood glucose levels compared with the db/db-control mice, with blood glucose levels decreasing within 120 min of the insulin injection.

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.

Materials/methods: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.

Results: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.

Conclusions: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

No MeSH data available.


Related in: MedlinePlus