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Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ- db/db mice

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.

Materials/methods: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.

Results: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.

Conclusions: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

No MeSH data available.


Related in: MedlinePlus

Weekly changes in blood glucose levels in C57BL/KsJ-db/db mice supplemented G. procumbens extract (GPE).db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group, a-c Mean values designed by different letters are significantly different between groups (P < 0.05).
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Figure 1: Weekly changes in blood glucose levels in C57BL/KsJ-db/db mice supplemented G. procumbens extract (GPE).db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group, a-c Mean values designed by different letters are significantly different between groups (P < 0.05).

Mentions: Fasting blood glucose levels in db/db-GPE group mice are shown in Fig. 1. At the beginning of the study, blood glucose levels were not significantly different between groups. However, fasting blood glucose levels of db/db-GPE mice after 4 weeks were significantly lower than those of the db/db-control mice. Blood glucose levels measured in db/db-control mice were elevated throughout the experiment, likely reflecting the progress of diabetes mellitus. As shown in Table 2, HbA1c values were 12.92 ± 0.31, 8.87 ± 1.09 and 7.45 ± 0.2 % in db/db-control, db/db-GPE and db/db-RG group, respectively. HbA1c values in the db/db-GPE mice were significantly lower than those measured in the db/db-control mice (P < 0.05).


Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ- db/db mice
Weekly changes in blood glucose levels in C57BL/KsJ-db/db mice supplemented G. procumbens extract (GPE).db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group, a-c Mean values designed by different letters are significantly different between groups (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037068&req=5

Figure 1: Weekly changes in blood glucose levels in C57BL/KsJ-db/db mice supplemented G. procumbens extract (GPE).db/db (diabetes mellitus control): C57BL/KsJ-db/db mice fed AIN-93G diet; db/db-RG: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with rosiglitazone (0.005 g/100 g diet); db/db-GPE: C57BL/KsJ-db/db mice fed AIN-93G diet supplemented with GPE (0.5 g/100 g diet). Values are presented as means ± SD, n = 7 per group, a-c Mean values designed by different letters are significantly different between groups (P < 0.05).
Mentions: Fasting blood glucose levels in db/db-GPE group mice are shown in Fig. 1. At the beginning of the study, blood glucose levels were not significantly different between groups. However, fasting blood glucose levels of db/db-GPE mice after 4 weeks were significantly lower than those of the db/db-control mice. Blood glucose levels measured in db/db-control mice were elevated throughout the experiment, likely reflecting the progress of diabetes mellitus. As shown in Table 2, HbA1c values were 12.92 ± 0.31, 8.87 ± 1.09 and 7.45 ± 0.2 % in db/db-control, db/db-GPE and db/db-RG group, respectively. HbA1c values in the db/db-GPE mice were significantly lower than those measured in the db/db-control mice (P < 0.05).

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: This study was designed to investigate whether Gynura procumbens extract (GPE) can improve insulin sensitivity and suppress hepatic glucose production in an animal model of type 2 diabetes.

Materials/methods: C57BL/Ksj-db/db mice were divided into 3 groups, a regular diet (control), GPE, and rosiglitazone groups (0.005 g/100 g diet) and fed for 6 weeks.

Results: Mice supplemented with GPE showed significantly lower blood levels of glucose and glycosylated hemoglobin than diabetic control mice. Glucose and insulin tolerance test also showed the positive effect of GPE on increasing insulin sensitivity. The homeostatic index of insulin resistance was significantly lower in mice supplemented with GPE than in the diabetic control mice. In the skeletal muscle, the expression of phosphorylated AMP-activated protein kinase, pAkt substrate of 160 kDa, and PM-glucose transporter type 4 increased in mice supplemented with GPE when compared to that of the diabetic control mice. GPE also decreased the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver.

Conclusions: These findings demonstrate that GPE might improve insulin sensitivity and inhibit gluconeogenesis in the liver.

No MeSH data available.


Related in: MedlinePlus