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Korean Curcuma longa L . induces lipolysis and regulates leptin in adipocyte cells and rats

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Turmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet.

Materials/methods: Leptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration.

Results: Contents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group.

Conclusions: Based on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

No MeSH data available.


Expression of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA from 3T3-L1 adipocytes stimulated by turmeric extracts.The effect of turmeric extracts on ATGL and HSL mRNA levels were investigated in 3T3-L1 adipocytes after 24 hours of treatment by RT-PCR analysis (A). Representative 2% agarose gel electrophoresis of PCR products stained with ethidium bromide. The right graphs show the mean band densitometry of ATGL or HSL/GAPDH relative expression.
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Figure 4: Expression of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA from 3T3-L1 adipocytes stimulated by turmeric extracts.The effect of turmeric extracts on ATGL and HSL mRNA levels were investigated in 3T3-L1 adipocytes after 24 hours of treatment by RT-PCR analysis (A). Representative 2% agarose gel electrophoresis of PCR products stained with ethidium bromide. The right graphs show the mean band densitometry of ATGL or HSL/GAPDH relative expression.

Mentions: To further define the lipolysis by turmeric extracts, ATGL and HSL of key enzymes involved in intracellular degradation of triglyceride are confirmed by gene expression (Fig. 4). Our results demonstrated as follows; mRNA levels of ATGL and HSL were increased in 3T3-L1 cells treated with turmeric extracts when compared to the untreated with the control group.


Korean Curcuma longa L . induces lipolysis and regulates leptin in adipocyte cells and rats
Expression of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA from 3T3-L1 adipocytes stimulated by turmeric extracts.The effect of turmeric extracts on ATGL and HSL mRNA levels were investigated in 3T3-L1 adipocytes after 24 hours of treatment by RT-PCR analysis (A). Representative 2% agarose gel electrophoresis of PCR products stained with ethidium bromide. The right graphs show the mean band densitometry of ATGL or HSL/GAPDH relative expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037065&req=5

Figure 4: Expression of hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA from 3T3-L1 adipocytes stimulated by turmeric extracts.The effect of turmeric extracts on ATGL and HSL mRNA levels were investigated in 3T3-L1 adipocytes after 24 hours of treatment by RT-PCR analysis (A). Representative 2% agarose gel electrophoresis of PCR products stained with ethidium bromide. The right graphs show the mean band densitometry of ATGL or HSL/GAPDH relative expression.
Mentions: To further define the lipolysis by turmeric extracts, ATGL and HSL of key enzymes involved in intracellular degradation of triglyceride are confirmed by gene expression (Fig. 4). Our results demonstrated as follows; mRNA levels of ATGL and HSL were increased in 3T3-L1 cells treated with turmeric extracts when compared to the untreated with the control group.

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Turmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet.

Materials/methods: Leptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration.

Results: Contents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group.

Conclusions: Based on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

No MeSH data available.