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Korean Curcuma longa L . induces lipolysis and regulates leptin in adipocyte cells and rats

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Turmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet.

Materials/methods: Leptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration.

Results: Contents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group.

Conclusions: Based on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

No MeSH data available.


Effects of turmeric extracts on viability of differentiating 3T3-L1 adipocytes.MTT colorimetric assay testing cell viability was performed after 24 hours of treatment with indicated concentration of turmeric. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05.
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Figure 1: Effects of turmeric extracts on viability of differentiating 3T3-L1 adipocytes.MTT colorimetric assay testing cell viability was performed after 24 hours of treatment with indicated concentration of turmeric. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05.

Mentions: The cytotoxicity of the turmeric in 3T3-L1 cells was evaluated to determine the test concentration for the further analysis. The cell viability is shown in Fig. 1. The cell viability in the concentration of turmerics, 2.5, 5, 10, 20 and 40 µg/ml was 100, 97.72, 90.97, 34.94 and 16.77%, respectively. The cell viability assay showed that the turmerics affect no damage at concentrations of 2.5, 5 and 10 µg/ml. Therefore, we used the concentration of turmeric up to 10 µg/ml. Fig. 2A shows the level of free fatty acids, and one of the product of lipolysis. Lipolysis is the process in which triglycerides are hydrolyzed into free fatty acids and glycerol. Free fatty acid was significantly increased by 76.36, 93.18 and 171.82% compared with controls when 3T3-L1 cells were treated with the 2.5, 5 and 10 µg/ml of turmeric, respectively. Glycerol also was significantly increased by 64.55, 88.48 and 155.9% compared with controls (Fig. 2B). The results indicate that the turmeric has a function of lipolysis.


Korean Curcuma longa L . induces lipolysis and regulates leptin in adipocyte cells and rats
Effects of turmeric extracts on viability of differentiating 3T3-L1 adipocytes.MTT colorimetric assay testing cell viability was performed after 24 hours of treatment with indicated concentration of turmeric. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037065&req=5

Figure 1: Effects of turmeric extracts on viability of differentiating 3T3-L1 adipocytes.MTT colorimetric assay testing cell viability was performed after 24 hours of treatment with indicated concentration of turmeric. All values are presented as means ± SE. (n≥3). *Statistical significance determined by Tukey's test at P < 0.05.
Mentions: The cytotoxicity of the turmeric in 3T3-L1 cells was evaluated to determine the test concentration for the further analysis. The cell viability is shown in Fig. 1. The cell viability in the concentration of turmerics, 2.5, 5, 10, 20 and 40 µg/ml was 100, 97.72, 90.97, 34.94 and 16.77%, respectively. The cell viability assay showed that the turmerics affect no damage at concentrations of 2.5, 5 and 10 µg/ml. Therefore, we used the concentration of turmeric up to 10 µg/ml. Fig. 2A shows the level of free fatty acids, and one of the product of lipolysis. Lipolysis is the process in which triglycerides are hydrolyzed into free fatty acids and glycerol. Free fatty acid was significantly increased by 76.36, 93.18 and 171.82% compared with controls when 3T3-L1 cells were treated with the 2.5, 5 and 10 µg/ml of turmeric, respectively. Glycerol also was significantly increased by 64.55, 88.48 and 155.9% compared with controls (Fig. 2B). The results indicate that the turmeric has a function of lipolysis.

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Turmeric (Curcuma longa L.) has been reported to have many biological functions including anti-obesity. Leptin, peptide hormone produced by adipocytes and its concentration is increased in proportion to the amount of the adipocytes. In the present study, we examined the effects of Korean turmeric on the regulation of adiposity and leptin levels in 3T3-L1 adipocytes and rats fed a high-fat and high-cholesterol diet.

Materials/methods: Leptin secretion, free fatty acid and glycerol contents in 3T3-L1 adipocytes were measured after incubation of cells with turmeric for 24 hours. Rats were divided into four experimental groups: a normal diet group (N), a high-fat and high-cholesterol diet group (HF), a high-fat and high-cholesterol diet group supplemented with 2.5% turmeric extracts (TPA group) and a high-fat and high-cholesterol diet group supplemented with 5% turmeric extracts (TPB group). Serum samples were used for the measurement of leptin concentration.

Results: Contents of free fatty acid and glycerol showed concentration dependent increase in response to turmeric extracts. Effects of turmeric extracts on reduction of lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Treatment with turmeric extracts resulted in increased expression levels of adipose triglyceride lipase and hormone-sensitive lipase mRNA. The concentration of leptin from 3T3-L1 adipocytes was significantly decreased by turmeric. Proportional abdominal and epididymal fats weights of the turmeric 5% supplemented group, TPB has significantly decreased compared to the HF group. The serum levels of leptin in the TPA and TPB groups were significantly lower than those of the HF group.

Conclusions: Based on these results, we suggested that Korean turmeric may contribute to the decreasing of body fat and regulating leptin secretion.

No MeSH data available.