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Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD.

Materials/methods: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR.

Results: In intestine, significantly lower Cd36 mRNA expression (P < 0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P < 0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly.

Conclusions: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

No MeSH data available.


Related in: MedlinePlus

The mRNA levels of genes related to intestinal chylomicron assembly and secretion.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05.
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Figure 2: The mRNA levels of genes related to intestinal chylomicron assembly and secretion.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05.

Mentions: To determine whether the effect of PNO on weight gain reduction was associated with changes in chylomicron secretion, we measured the expression of genes involved in intestinal chylomicron assembly and secretion. The mRNA level of Mtp, an enzyme involved in chylomicron synthesis and secretion, was significantly upregulated in HFD-fed mice (P < 0.05). The PHF group tended to have a lower Mtp mRNA level than the SHF group (0.81-fold, P = 0.08). The structural components of gene expression of chylomicron, ApoB48 and ApoA4, which also facilitate intestinal lipoprotein production, were measured. Although the results of ANOVA didn't show a significant difference in the mRNA levels of ApoA4, overall tendency of lower expression was observed in PNO-fed mice compared with SBO fed mice (P = 0.07). In individual group comparisons, significantly lower mRNA levels of ApoA4 were observed in the PHF group than in the SHF group (0.44-fold, P < 0.05). Neither fat amount nor oil type influenced mRNA levels of ApoB48 (Fig. 2).


Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice
The mRNA levels of genes related to intestinal chylomicron assembly and secretion.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037064&req=5

Figure 2: The mRNA levels of genes related to intestinal chylomicron assembly and secretion.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05.
Mentions: To determine whether the effect of PNO on weight gain reduction was associated with changes in chylomicron secretion, we measured the expression of genes involved in intestinal chylomicron assembly and secretion. The mRNA level of Mtp, an enzyme involved in chylomicron synthesis and secretion, was significantly upregulated in HFD-fed mice (P < 0.05). The PHF group tended to have a lower Mtp mRNA level than the SHF group (0.81-fold, P = 0.08). The structural components of gene expression of chylomicron, ApoB48 and ApoA4, which also facilitate intestinal lipoprotein production, were measured. Although the results of ANOVA didn't show a significant difference in the mRNA levels of ApoA4, overall tendency of lower expression was observed in PNO-fed mice compared with SBO fed mice (P = 0.07). In individual group comparisons, significantly lower mRNA levels of ApoA4 were observed in the PHF group than in the SHF group (0.44-fold, P < 0.05). Neither fat amount nor oil type influenced mRNA levels of ApoB48 (Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD.

Materials/methods: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR.

Results: In intestine, significantly lower Cd36 mRNA expression (P &lt; 0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P &lt; 0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly.

Conclusions: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

No MeSH data available.


Related in: MedlinePlus