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Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD.

Materials/methods: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR.

Results: In intestine, significantly lower Cd36 mRNA expression (P < 0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P < 0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly.

Conclusions: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

No MeSH data available.


Related in: MedlinePlus

The mRNA levels of genes related to intestinal fatty acid uptake and channeling.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05. *A significant overall effect of fat amount was observed in a two-way ANOVA, but no significant differences were observed among individual group comparisons in the post hoc test.
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Figure 1: The mRNA levels of genes related to intestinal fatty acid uptake and channeling.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05. *A significant overall effect of fat amount was observed in a two-way ANOVA, but no significant differences were observed among individual group comparisons in the post hoc test.

Mentions: To examine the effect of PNO replacement in diet on intestinal lipid absorption, the expression of genes related to fatty acid absorption, Cd36 and Fatp4, and the genes involved in fatty acid channeling within enterocyte, Acsl5 and Acbp, were measured (Fig. 1). Overall, the mRNA levels of Cd36 were significantly higher in HFD-fed mice (P < 0.05) but significantly lower in PNO-fed mice (P < 0.05). The PHF group showed a tendency of lower Cd36 mRNA level than the SHF group (0.64-fold, P = 0.06). Feeding HFD resulted in a significantly higher Acbp gene expression (P < 0.05), but no statistical difference was observed regarding different oil type. The mRNA levels of Fatp4 and Acsl5 were not influenced by amount of fat and type of oil.


Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice
The mRNA levels of genes related to intestinal fatty acid uptake and channeling.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05. *A significant overall effect of fat amount was observed in a two-way ANOVA, but no significant differences were observed among individual group comparisons in the post hoc test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037064&req=5

Figure 1: The mRNA levels of genes related to intestinal fatty acid uptake and channeling.Data are presented as mean ± SEM, n = 6 for each group. Two-way ANOVA was used to determine the significant effects of fat amount and oil type and was followed by a LSD post-hoc test. Different letters indicate significant difference at P < 0.05. *A significant overall effect of fat amount was observed in a two-way ANOVA, but no significant differences were observed among individual group comparisons in the post hoc test.
Mentions: To examine the effect of PNO replacement in diet on intestinal lipid absorption, the expression of genes related to fatty acid absorption, Cd36 and Fatp4, and the genes involved in fatty acid channeling within enterocyte, Acsl5 and Acbp, were measured (Fig. 1). Overall, the mRNA levels of Cd36 were significantly higher in HFD-fed mice (P < 0.05) but significantly lower in PNO-fed mice (P < 0.05). The PHF group showed a tendency of lower Cd36 mRNA level than the SHF group (0.64-fold, P = 0.06). Feeding HFD resulted in a significantly higher Acbp gene expression (P < 0.05), but no statistical difference was observed regarding different oil type. The mRNA levels of Fatp4 and Acsl5 were not influenced by amount of fat and type of oil.

View Article: PubMed Central - PubMed

ABSTRACT

Background/objectives: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD.

Materials/methods: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR.

Results: In intestine, significantly lower Cd36 mRNA expression (P &lt; 0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P &lt; 0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly.

Conclusions: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

No MeSH data available.


Related in: MedlinePlus