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Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.


The mRNA and protein levels of Abca13, Grb2, Ptgds in MCAO group and MCAO + MSCs group at indicated time points. The mRNA and protein levels of Abca13 are shown in (A) and (B), respectively. The mRNA and protein levels of Grb2 are shown in (C) and (D), respectively. The mRNA and protein levels of Ptgds are shown in (E) and (F), respectively. *p<0.05; **p<0.01 compared with sham group; #p<0.05; ##p<0.01 compared with MCAO group.
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F5-ad-7-5-623: The mRNA and protein levels of Abca13, Grb2, Ptgds in MCAO group and MCAO + MSCs group at indicated time points. The mRNA and protein levels of Abca13 are shown in (A) and (B), respectively. The mRNA and protein levels of Grb2 are shown in (C) and (D), respectively. The mRNA and protein levels of Ptgds are shown in (E) and (F), respectively. *p<0.05; **p<0.01 compared with sham group; #p<0.05; ##p<0.01 compared with MCAO group.

Mentions: Abca13: At the mRNA level, the Abca13 in the MCAO group was decreased at all time points compared with control group. Abca13 was at its lowest level at 6 h after I/R, and then reached its peak at 48 h after I/R and decreased after 1 week. However, after treatment with hUC-MSCs, Abca13 was increased at 24 h and 48 h after I/R compared with the MCAO group (Fig. 5A). At the protein level, Abca13 was up-regulated in the MCAO group at all the time points compared with the control group. When treated with hUC-MSCs, Abca13 was down-regulated at 24 h and up-regulated at 72 h after I/R compared with the MCAO group (Fig. 5B).


Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke
The mRNA and protein levels of Abca13, Grb2, Ptgds in MCAO group and MCAO + MSCs group at indicated time points. The mRNA and protein levels of Abca13 are shown in (A) and (B), respectively. The mRNA and protein levels of Grb2 are shown in (C) and (D), respectively. The mRNA and protein levels of Ptgds are shown in (E) and (F), respectively. *p<0.05; **p<0.01 compared with sham group; #p<0.05; ##p<0.01 compared with MCAO group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036957&req=5

F5-ad-7-5-623: The mRNA and protein levels of Abca13, Grb2, Ptgds in MCAO group and MCAO + MSCs group at indicated time points. The mRNA and protein levels of Abca13 are shown in (A) and (B), respectively. The mRNA and protein levels of Grb2 are shown in (C) and (D), respectively. The mRNA and protein levels of Ptgds are shown in (E) and (F), respectively. *p<0.05; **p<0.01 compared with sham group; #p<0.05; ##p<0.01 compared with MCAO group.
Mentions: Abca13: At the mRNA level, the Abca13 in the MCAO group was decreased at all time points compared with control group. Abca13 was at its lowest level at 6 h after I/R, and then reached its peak at 48 h after I/R and decreased after 1 week. However, after treatment with hUC-MSCs, Abca13 was increased at 24 h and 48 h after I/R compared with the MCAO group (Fig. 5A). At the protein level, Abca13 was up-regulated in the MCAO group at all the time points compared with the control group. When treated with hUC-MSCs, Abca13 was down-regulated at 24 h and up-regulated at 72 h after I/R compared with the MCAO group (Fig. 5B).

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.