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Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.


The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differentially expressed proteins in the four experimental groups. The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differential expression in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C) and the mcao-48h + MSCs/mcao-48h group (D) are shown. The transcription factors of c-myb, Runx-1, Pu.1, Irf8, and Hoxb8 are represented by myb, runx1, Spi1, Irf8 and Hoxb8, respectively.
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F4-ad-7-5-623: The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differentially expressed proteins in the four experimental groups. The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differential expression in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C) and the mcao-48h + MSCs/mcao-48h group (D) are shown. The transcription factors of c-myb, Runx-1, Pu.1, Irf8, and Hoxb8 are represented by myb, runx1, Spi1, Irf8 and Hoxb8, respectively.

Mentions: We then analyzed the relationship between transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) and differentially expressed proteins in the four groups (Fig. 4A-4D) The transcription factors of c-myb, Runx-1, Pu.1, Irf8, and Hoxb8 are represented by myb, runx1, Spi1, Irf8, and Hoxb8, respectively. We found that Grb2 is located at the center of the network.


Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke
The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differentially expressed proteins in the four experimental groups. The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differential expression in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C) and the mcao-48h + MSCs/mcao-48h group (D) are shown. The transcription factors of c-myb, Runx-1, Pu.1, Irf8, and Hoxb8 are represented by myb, runx1, Spi1, Irf8 and Hoxb8, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036957&req=5

F4-ad-7-5-623: The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differentially expressed proteins in the four experimental groups. The network with transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) of differential expression in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C) and the mcao-48h + MSCs/mcao-48h group (D) are shown. The transcription factors of c-myb, Runx-1, Pu.1, Irf8, and Hoxb8 are represented by myb, runx1, Spi1, Irf8 and Hoxb8, respectively.
Mentions: We then analyzed the relationship between transcription factors (c-myb, Runx-1, Pu.1, Irf8, Hoxb8) and differentially expressed proteins in the four groups (Fig. 4A-4D) The transcription factors of c-myb, Runx-1, Pu.1, Irf8, and Hoxb8 are represented by myb, runx1, Spi1, Irf8, and Hoxb8, respectively. We found that Grb2 is located at the center of the network.

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.