Limits...
Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.


The network associated with CD200-CD200R1, CCL21-CXCR3 of differentially expressed proteins in the four experimental groups. The network with CD200-CD200R1 and CCL21-CXCR3 of differentially expressed proteins in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D) are shown. Note that the trend in the mcao-24h/sham group was almost opposite to that of the mcao-48h/mcao-24h group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5036957&req=5

F3-ad-7-5-623: The network associated with CD200-CD200R1, CCL21-CXCR3 of differentially expressed proteins in the four experimental groups. The network with CD200-CD200R1 and CCL21-CXCR3 of differentially expressed proteins in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D) are shown. Note that the trend in the mcao-24h/sham group was almost opposite to that of the mcao-48h/mcao-24h group.

Mentions: The analysis of relationship between key molecules associated with neuron-glial crosstalk (CD200-CD200R1, CCL21-CXCR3) and differentially expressed proteins of these four groups are shown in Fig. 3A - 3D.


Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke
The network associated with CD200-CD200R1, CCL21-CXCR3 of differentially expressed proteins in the four experimental groups. The network with CD200-CD200R1 and CCL21-CXCR3 of differentially expressed proteins in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D) are shown. Note that the trend in the mcao-24h/sham group was almost opposite to that of the mcao-48h/mcao-24h group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036957&req=5

F3-ad-7-5-623: The network associated with CD200-CD200R1, CCL21-CXCR3 of differentially expressed proteins in the four experimental groups. The network with CD200-CD200R1 and CCL21-CXCR3 of differentially expressed proteins in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D) are shown. Note that the trend in the mcao-24h/sham group was almost opposite to that of the mcao-48h/mcao-24h group.
Mentions: The analysis of relationship between key molecules associated with neuron-glial crosstalk (CD200-CD200R1, CCL21-CXCR3) and differentially expressed proteins of these four groups are shown in Fig. 3A - 3D.

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.