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Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.


The GO term analysis of differentially expressed proteins in the four experimental groups. The GO term analysis of differentially expressed protein in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D). Every GO term analysis shows genes involved in cellular component, molecular function and biological process.
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F2-ad-7-5-623: The GO term analysis of differentially expressed proteins in the four experimental groups. The GO term analysis of differentially expressed protein in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D). Every GO term analysis shows genes involved in cellular component, molecular function and biological process.

Mentions: In the Gene Ontology (GO) analysis, a gene or protein could be annotated in 3 ways: cellular component, molecular function, and biological process. An analysis of GO was performed to determine the differentially expressed proteins in the four experimental groups. In the mcao-24h/sham group, the differentially expressed proteins were involved in cellular component: cell membrane, cytoplasm, nucleus and organelle; in molecular function: binding and catalytic; and in biological process: biological regulation, cellular process, metabolic process and pigmentation (Fig. 2A). In the mcao-48h/sham group, the differentially expressed proteins were involved in molecular function: binding and catalytic; and in biological process: cellular process and localization (Fig. 2B). In the mcao-48h/mcao-24h group, the differentially expressed proteins were involved in cellular component: cell membrane, cytoplasm, nucleus and organelle; in molecular function: binding, catalytic and structural molecule; and in biological process: biological regulation, cellular process, localization, metabolic process and pigmentation (Fig. 2C). In the mcao-48h + MSCs/mcao-48h group, the differentially expressed proteins were involved in cellular component: cell membrane, cytoplasm, nucleus and organelle; in molecular function: binding; and in biological process: biological regulation, cellular process, establishment of localization, localization, metabolic process, multicellular organismal process and pigmentation (Fig. 2D).


Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke
The GO term analysis of differentially expressed proteins in the four experimental groups. The GO term analysis of differentially expressed protein in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D). Every GO term analysis shows genes involved in cellular component, molecular function and biological process.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036957&req=5

F2-ad-7-5-623: The GO term analysis of differentially expressed proteins in the four experimental groups. The GO term analysis of differentially expressed protein in the mcao-24h/sham group (A), the mcao-48h/sham group (B), the mcao-48h/mcao-24h group (C), and the mcao-48h + MSCs/mcao-48h group (D). Every GO term analysis shows genes involved in cellular component, molecular function and biological process.
Mentions: In the Gene Ontology (GO) analysis, a gene or protein could be annotated in 3 ways: cellular component, molecular function, and biological process. An analysis of GO was performed to determine the differentially expressed proteins in the four experimental groups. In the mcao-24h/sham group, the differentially expressed proteins were involved in cellular component: cell membrane, cytoplasm, nucleus and organelle; in molecular function: binding and catalytic; and in biological process: biological regulation, cellular process, metabolic process and pigmentation (Fig. 2A). In the mcao-48h/sham group, the differentially expressed proteins were involved in molecular function: binding and catalytic; and in biological process: cellular process and localization (Fig. 2B). In the mcao-48h/mcao-24h group, the differentially expressed proteins were involved in cellular component: cell membrane, cytoplasm, nucleus and organelle; in molecular function: binding, catalytic and structural molecule; and in biological process: biological regulation, cellular process, localization, metabolic process and pigmentation (Fig. 2C). In the mcao-48h + MSCs/mcao-48h group, the differentially expressed proteins were involved in cellular component: cell membrane, cytoplasm, nucleus and organelle; in molecular function: binding; and in biological process: biological regulation, cellular process, establishment of localization, localization, metabolic process, multicellular organismal process and pigmentation (Fig. 2D).

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.