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Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.


Related in: MedlinePlus

The number of overlapping proteins in the four groups. The blue circle represent the up-regulated and down-regulated proteins at 24 hours after cerebral ischemia compared to sham group, the yellow circle represent the up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared to sham group, the green circle represent up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared with the 24 hours after cerebral ischemia group, the red circle represent up-regulated and down-regulated proteins after hUC-MSCs treatment compared with the 48 hours after cerebral ischemia group.
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F1-ad-7-5-623: The number of overlapping proteins in the four groups. The blue circle represent the up-regulated and down-regulated proteins at 24 hours after cerebral ischemia compared to sham group, the yellow circle represent the up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared to sham group, the green circle represent up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared with the 24 hours after cerebral ischemia group, the red circle represent up-regulated and down-regulated proteins after hUC-MSCs treatment compared with the 48 hours after cerebral ischemia group.

Mentions: We found that Acat2, Grb2, and Scp2 were down-regulated in the mcao-24h group compared with the sham group and up-regulated in the mcao-48h group compared with the mcao-24h group. On the other hand, Ptgds and Hbb-b1 were up-regulated in the mcao-24h group compared with sham group and down-regulated in the mcao-48h group compared with the mcao-24h group. We also found that Rrbp1, Anxa6, Slk, Nup205 and Psmd6 changed in the mcao-48h + hUC-MSC group only compared with the mcao-48h group. Detailed protein expressions in each group are listed in Table 2. A Venn diagram is used to show the relationship of differentially expressed proteins in these groups (Fig. 1).


Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke
The number of overlapping proteins in the four groups. The blue circle represent the up-regulated and down-regulated proteins at 24 hours after cerebral ischemia compared to sham group, the yellow circle represent the up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared to sham group, the green circle represent up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared with the 24 hours after cerebral ischemia group, the red circle represent up-regulated and down-regulated proteins after hUC-MSCs treatment compared with the 48 hours after cerebral ischemia group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036957&req=5

F1-ad-7-5-623: The number of overlapping proteins in the four groups. The blue circle represent the up-regulated and down-regulated proteins at 24 hours after cerebral ischemia compared to sham group, the yellow circle represent the up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared to sham group, the green circle represent up-regulated and down-regulated proteins at 48 hours after cerebral ischemia compared with the 24 hours after cerebral ischemia group, the red circle represent up-regulated and down-regulated proteins after hUC-MSCs treatment compared with the 48 hours after cerebral ischemia group.
Mentions: We found that Acat2, Grb2, and Scp2 were down-regulated in the mcao-24h group compared with the sham group and up-regulated in the mcao-48h group compared with the mcao-24h group. On the other hand, Ptgds and Hbb-b1 were up-regulated in the mcao-24h group compared with sham group and down-regulated in the mcao-48h group compared with the mcao-24h group. We also found that Rrbp1, Anxa6, Slk, Nup205 and Psmd6 changed in the mcao-48h + hUC-MSC group only compared with the mcao-48h group. Detailed protein expressions in each group are listed in Table 2. A Venn diagram is used to show the relationship of differentially expressed proteins in these groups (Fig. 1).

View Article: PubMed Central - PubMed

ABSTRACT

Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.

No MeSH data available.


Related in: MedlinePlus