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Serum IL-33 Is a Novel Diagnostic and Prognostic Biomarker in Acute Ischemic Stroke

View Article: PubMed Central - PubMed

ABSTRACT

Interleukin-33 (IL-33), a newly recognized IL-1 family member, is expressed in various tissues and cells, and involved in pathogenesis of many human diseases. For example, IL-33 plays a protective role in cardiovascular diseases. However, the role of IL-33 in acute ischemic stroke (AIS) remains unclear. This study aims to investigate whether IL-33 level in AIS patient serum can be used as a potential diagnostic and prognostic marker. The study included two hundred and six patients with first-ever ischemic stroke, who were admitted within 72 hours after stroke onset. The serum level of IL-33 was measured with ELISA and the severity of AIS patients on admission was evaluated based on the National Institutes of Health Stroke Scale (NIHSS) score. The functional outcome at 3 months was determined using the Barthel index (BI). We found that serum IL-33 was significantly higher (P < 0.001) in patients with AIS [57.68 ng/L (IQR, 44.95-76.73)] compared with healthy controls [47.48 ng/L (IQR, 38.67-53.78)]. IL-33 was an independent diagnostic biomarker for AIS with an OR of 1.051 (95%Cl, 1.018-1.085; P=0.002). Serum IL-33 was higher (P < 0.05) in the stroke patients with small cerebral infarction volume compared to AIS patients with large cerebral infarction. In addition, serum IL-33 was also significantly higher (P = 0.001) in the patients with mild stroke, compared to the patients with severe stroke. Furthermore, serum IL-33 level in AIS patients with a worse outcome was higher (P < 0.001) compared to AIS patients with a better outcome. IL-33 was also an independent predictor for the functional outcome with an adjusted OR of 0.932 (95% CI, 0.882-0.986). Our results suggest that the lower level of serum IL-33 is associated with large infarction volume and greater stroke severity in AIS patients. Thus, IL-33 can be used as a novel and independent diagnostic and predicting prognostic marker in AIS.

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Serum levels of IL-33 in different groups. (A) Serum levels of IL-33 in IAS Patients and healthy controls. (B) Serum levels of IL-33 in small and large infarct volume groups. Statistical comparisons were made using the Mann-Whitney test. A:P < 0.001; B:P < 0.05.
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F1-ad-7-5-614: Serum levels of IL-33 in different groups. (A) Serum levels of IL-33 in IAS Patients and healthy controls. (B) Serum levels of IL-33 in small and large infarct volume groups. Statistical comparisons were made using the Mann-Whitney test. A:P < 0.001; B:P < 0.05.

Mentions: We found the median serum IL-33 concentration was significantly higher (P < 0.001) in AIS patients compared to healthy controls (Fig. 1A). There was a weak but significant negative correlation between IL-33 and Hs-CRP (r = -0.178, P=0.011). There was no effect of age, sex, risk factors of stroke, WBC, glucose, lithic acid, TG, TC, HDL, LDL, IgG, IgA, IgM, and HbA1c on IL-33 on AIS patients (P>0.05 for all categories).


Serum IL-33 Is a Novel Diagnostic and Prognostic Biomarker in Acute Ischemic Stroke
Serum levels of IL-33 in different groups. (A) Serum levels of IL-33 in IAS Patients and healthy controls. (B) Serum levels of IL-33 in small and large infarct volume groups. Statistical comparisons were made using the Mann-Whitney test. A:P < 0.001; B:P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036956&req=5

F1-ad-7-5-614: Serum levels of IL-33 in different groups. (A) Serum levels of IL-33 in IAS Patients and healthy controls. (B) Serum levels of IL-33 in small and large infarct volume groups. Statistical comparisons were made using the Mann-Whitney test. A:P < 0.001; B:P < 0.05.
Mentions: We found the median serum IL-33 concentration was significantly higher (P < 0.001) in AIS patients compared to healthy controls (Fig. 1A). There was a weak but significant negative correlation between IL-33 and Hs-CRP (r = -0.178, P=0.011). There was no effect of age, sex, risk factors of stroke, WBC, glucose, lithic acid, TG, TC, HDL, LDL, IgG, IgA, IgM, and HbA1c on IL-33 on AIS patients (P>0.05 for all categories).

View Article: PubMed Central - PubMed

ABSTRACT

Interleukin-33 (IL-33), a newly recognized IL-1 family member, is expressed in various tissues and cells, and involved in pathogenesis of many human diseases. For example, IL-33 plays a protective role in cardiovascular diseases. However, the role of IL-33 in acute ischemic stroke (AIS) remains unclear. This study aims to investigate whether IL-33 level in AIS patient serum can be used as a potential diagnostic and prognostic marker. The study included two hundred and six patients with first-ever ischemic stroke, who were admitted within 72 hours after stroke onset. The serum level of IL-33 was measured with ELISA and the severity of AIS patients on admission was evaluated based on the National Institutes of Health Stroke Scale (NIHSS) score. The functional outcome at 3 months was determined using the Barthel index (BI). We found that serum IL-33 was significantly higher (P &lt; 0.001) in patients with AIS [57.68 ng/L (IQR, 44.95-76.73)] compared with healthy controls [47.48 ng/L (IQR, 38.67-53.78)]. IL-33 was an independent diagnostic biomarker for AIS with an OR of 1.051 (95%Cl, 1.018-1.085; P=0.002). Serum IL-33 was higher (P &lt; 0.05) in the stroke patients with small cerebral infarction volume compared to AIS patients with large cerebral infarction. In addition, serum IL-33 was also significantly higher (P = 0.001) in the patients with mild stroke, compared to the patients with severe stroke. Furthermore, serum IL-33 level in AIS patients with a worse outcome was higher (P &lt; 0.001) compared to AIS patients with a better outcome. IL-33 was also an independent predictor for the functional outcome with an adjusted OR of 0.932 (95% CI, 0.882-0.986). Our results suggest that the lower level of serum IL-33 is associated with large infarction volume and greater stroke severity in AIS patients. Thus, IL-33 can be used as a novel and independent diagnostic and predicting prognostic marker in AIS.

No MeSH data available.


Related in: MedlinePlus