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Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.


Comparison of telomere length in the circulating leukocytes in the peripheral blood of OSA patients (N = 43) depends on OSA severity. The telomere length of circulating leukocytes was significantly shorter in patients with severe OSA than patients with mild OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients). Graphs show mean values, and *p < .05 compared to patients with mild OSA (A). Telomere length of circulating leukocytes in OSA patients with >20 ODI was significantly shorter than in OSA patients with <10 ODI (B). Graphs show mean value + standard deviation (SD). *p < .05 compared to OSA patients with <10 ODI.
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F6-ad-7-5-604: Comparison of telomere length in the circulating leukocytes in the peripheral blood of OSA patients (N = 43) depends on OSA severity. The telomere length of circulating leukocytes was significantly shorter in patients with severe OSA than patients with mild OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients). Graphs show mean values, and *p < .05 compared to patients with mild OSA (A). Telomere length of circulating leukocytes in OSA patients with >20 ODI was significantly shorter than in OSA patients with <10 ODI (B). Graphs show mean value + standard deviation (SD). *p < .05 compared to OSA patients with <10 ODI.

Mentions: A significant inverse correlation was observed between telomere length and the RDI of forty-three OSA patients (R2=0.446, p < 0.05, Fig. 5). The mean telomere length in samples from the eleven patients with mild OSA was 1.40 ± 0.8; this value was significantly decreased in patients with severe OSA (1.07 ± 0.5, p < 0.05, Fig. 6A). OSA patients were also classified by oxygen desaturation index (ODI) dependence, and mean values of telomere length were compared according to ODI. Twelve OSA patients had an ODI lower than 10; eighteen had an ODI from 10 to 20; and thirteen had an ODI greater than 20. The mean telomere length was shorter in patients with an ODI greater than 20 compared to patients with an ODI less than 10 (0.89 ± 0.5 greater than 20 vs. 1.75 ± 0.6 less than 10; Fig. 6B). These results revealed that telomere shortening is related to OSA severity as shorter telomere lengths were seen in OSA patients with a greater number of apnea events and lower oxygen desaturation indexes.


Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea
Comparison of telomere length in the circulating leukocytes in the peripheral blood of OSA patients (N = 43) depends on OSA severity. The telomere length of circulating leukocytes was significantly shorter in patients with severe OSA than patients with mild OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients). Graphs show mean values, and *p < .05 compared to patients with mild OSA (A). Telomere length of circulating leukocytes in OSA patients with >20 ODI was significantly shorter than in OSA patients with <10 ODI (B). Graphs show mean value + standard deviation (SD). *p < .05 compared to OSA patients with <10 ODI.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036955&req=5

F6-ad-7-5-604: Comparison of telomere length in the circulating leukocytes in the peripheral blood of OSA patients (N = 43) depends on OSA severity. The telomere length of circulating leukocytes was significantly shorter in patients with severe OSA than patients with mild OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients). Graphs show mean values, and *p < .05 compared to patients with mild OSA (A). Telomere length of circulating leukocytes in OSA patients with >20 ODI was significantly shorter than in OSA patients with <10 ODI (B). Graphs show mean value + standard deviation (SD). *p < .05 compared to OSA patients with <10 ODI.
Mentions: A significant inverse correlation was observed between telomere length and the RDI of forty-three OSA patients (R2=0.446, p < 0.05, Fig. 5). The mean telomere length in samples from the eleven patients with mild OSA was 1.40 ± 0.8; this value was significantly decreased in patients with severe OSA (1.07 ± 0.5, p < 0.05, Fig. 6A). OSA patients were also classified by oxygen desaturation index (ODI) dependence, and mean values of telomere length were compared according to ODI. Twelve OSA patients had an ODI lower than 10; eighteen had an ODI from 10 to 20; and thirteen had an ODI greater than 20. The mean telomere length was shorter in patients with an ODI greater than 20 compared to patients with an ODI less than 10 (0.89 ± 0.5 greater than 20 vs. 1.75 ± 0.6 less than 10; Fig. 6B). These results revealed that telomere shortening is related to OSA severity as shorter telomere lengths were seen in OSA patients with a greater number of apnea events and lower oxygen desaturation indexes.

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.