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Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.


Change in telomere length by body mass index (BMI). Mean telomere length diminished with higher BMI, and the lowest value was observed for BMI > 25 cm2/kg in healthy volunteers (eleven were less than 22.9 kg/cm2, thirteen were 23.0 to 24.9 kg/cm2, and ten were greater than 25.0 kg/cm2) (A). Mean values were significantly shorter in OSA patients (twelve were less than 22.9 kg/cm2, seventeen were 23.0 to 24.9 kg/cm2, and fourteen were greater than 25.0 kg/cm2) than in healthy volunteers in the same BMI range (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients. *p <.05 comparing healthy volunteers and OSA patients.
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F4-ad-7-5-604: Change in telomere length by body mass index (BMI). Mean telomere length diminished with higher BMI, and the lowest value was observed for BMI > 25 cm2/kg in healthy volunteers (eleven were less than 22.9 kg/cm2, thirteen were 23.0 to 24.9 kg/cm2, and ten were greater than 25.0 kg/cm2) (A). Mean values were significantly shorter in OSA patients (twelve were less than 22.9 kg/cm2, seventeen were 23.0 to 24.9 kg/cm2, and fourteen were greater than 25.0 kg/cm2) than in healthy volunteers in the same BMI range (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients. *p <.05 comparing healthy volunteers and OSA patients.

Mentions: The mean telomere length was 2.02 ± 0.9 in those with a BMI less than 22.9 kg/cm2 (N=11), 2.05 ± 0.8 in people with a BMI 23.0 to 24.9 kg/cm2 (N=13), and 1.48 ± 0.5 in people with a BMI greater than 25.0 kg/cm2 (N=10) (Fig. 4A). Thus, we found that telomere length from the whole blood DNA of healthy volunteers also shortened if subjects had greater BMI.


Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea
Change in telomere length by body mass index (BMI). Mean telomere length diminished with higher BMI, and the lowest value was observed for BMI > 25 cm2/kg in healthy volunteers (eleven were less than 22.9 kg/cm2, thirteen were 23.0 to 24.9 kg/cm2, and ten were greater than 25.0 kg/cm2) (A). Mean values were significantly shorter in OSA patients (twelve were less than 22.9 kg/cm2, seventeen were 23.0 to 24.9 kg/cm2, and fourteen were greater than 25.0 kg/cm2) than in healthy volunteers in the same BMI range (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients. *p <.05 comparing healthy volunteers and OSA patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036955&req=5

F4-ad-7-5-604: Change in telomere length by body mass index (BMI). Mean telomere length diminished with higher BMI, and the lowest value was observed for BMI > 25 cm2/kg in healthy volunteers (eleven were less than 22.9 kg/cm2, thirteen were 23.0 to 24.9 kg/cm2, and ten were greater than 25.0 kg/cm2) (A). Mean values were significantly shorter in OSA patients (twelve were less than 22.9 kg/cm2, seventeen were 23.0 to 24.9 kg/cm2, and fourteen were greater than 25.0 kg/cm2) than in healthy volunteers in the same BMI range (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients. *p <.05 comparing healthy volunteers and OSA patients.
Mentions: The mean telomere length was 2.02 ± 0.9 in those with a BMI less than 22.9 kg/cm2 (N=11), 2.05 ± 0.8 in people with a BMI 23.0 to 24.9 kg/cm2 (N=13), and 1.48 ± 0.5 in people with a BMI greater than 25.0 kg/cm2 (N=10) (Fig. 4A). Thus, we found that telomere length from the whole blood DNA of healthy volunteers also shortened if subjects had greater BMI.

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.