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Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.


Correlation between the concentration of hydrogen peroxide and telomere length in OSA patients. A significant inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of 43 OSA patients (R2=0.490) (A) and mean telomere length in healthy volunteers (N = 34) and OSA patients (N = 43) (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients (*p < .05 comparing healthy volunteers and OSA patients).
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F2-ad-7-5-604: Correlation between the concentration of hydrogen peroxide and telomere length in OSA patients. A significant inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of 43 OSA patients (R2=0.490) (A) and mean telomere length in healthy volunteers (N = 34) and OSA patients (N = 43) (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients (*p < .05 comparing healthy volunteers and OSA patients).

Mentions: We compared the change in telomere length with the hydrogen peroxide concentration of the peripheral blood in OSA patients to investigate potential associations between the two factors. Interestingly, we found a predictable inverse correlation between hydrogen peroxide concentration in the peripheral blood and telomere length of circulating leukocytes (R2=0.490, Fig. 2A). As a next step, we measured telomere length in circulating leukocytes from patients with OSA and healthy volunteers and the results showed that mean telomere length was significantly shorter in the DNA from whole blood samples of OSA patients compared to healthy volunteers (1.87 ± 0.74 vs. 1.14 ± 0.60, p < 0.05) (Fig. 2B). Then, we analyzed the change in telomere length in an age- and BMI-dependent manner, because telomere length is also influenced by age and body mass index (BMI). Telomere length of circulating leukocyte in whole blood DNA of healthy volunteers shortened in accordance with advancing subject’s age. Mean telomere length was 2.69 ± 0.7 in circulating leukocytes of healthy volunteers younger than 20 years (N=6), 2.04 ± 0.8 in healthy volunteers aged 20-40 years (N=12), 1.89 ± 0.4 in healthy volunteers aged 40-60 years (N=10), and 1.17 ± 0.4 in healthy volunteers older than 60 years (N=6) (Fig. 3A).


Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea
Correlation between the concentration of hydrogen peroxide and telomere length in OSA patients. A significant inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of 43 OSA patients (R2=0.490) (A) and mean telomere length in healthy volunteers (N = 34) and OSA patients (N = 43) (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients (*p < .05 comparing healthy volunteers and OSA patients).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036955&req=5

F2-ad-7-5-604: Correlation between the concentration of hydrogen peroxide and telomere length in OSA patients. A significant inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of 43 OSA patients (R2=0.490) (A) and mean telomere length in healthy volunteers (N = 34) and OSA patients (N = 43) (B). Graphs show mean values. White dot: telomere length of healthy volunteers; black dot: telomere length of OSA patients (*p < .05 comparing healthy volunteers and OSA patients).
Mentions: We compared the change in telomere length with the hydrogen peroxide concentration of the peripheral blood in OSA patients to investigate potential associations between the two factors. Interestingly, we found a predictable inverse correlation between hydrogen peroxide concentration in the peripheral blood and telomere length of circulating leukocytes (R2=0.490, Fig. 2A). As a next step, we measured telomere length in circulating leukocytes from patients with OSA and healthy volunteers and the results showed that mean telomere length was significantly shorter in the DNA from whole blood samples of OSA patients compared to healthy volunteers (1.87 ± 0.74 vs. 1.14 ± 0.60, p < 0.05) (Fig. 2B). Then, we analyzed the change in telomere length in an age- and BMI-dependent manner, because telomere length is also influenced by age and body mass index (BMI). Telomere length of circulating leukocyte in whole blood DNA of healthy volunteers shortened in accordance with advancing subject’s age. Mean telomere length was 2.69 ± 0.7 in circulating leukocytes of healthy volunteers younger than 20 years (N=6), 2.04 ± 0.8 in healthy volunteers aged 20-40 years (N=12), 1.89 ± 0.4 in healthy volunteers aged 40-60 years (N=10), and 1.17 ± 0.4 in healthy volunteers older than 60 years (N=6) (Fig. 3A).

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.