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Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.


The concentration of hydrogen peroxide in the peripheral blood of healthy volunteers (N = 34) and OSA patients (N = 43) and correlation with severity of OSA. Mean concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA patients (A). Mean concentration of hydrogen peroxide in the peripheral blood of OSA patients compared by severity of OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients) (B) and a significant correlation was observed between the concentration of hydrogen peroxide and the RDI of 43 OSA patients (R2=0.615) (C). Graphs show mean values. White dot: the concentration of hydrogen peroxide in healthy volunteers; black dot: the concentration of hydrogen peroxide in OSA patients (*p < .05 comparing healthy volunteers and OSA patients).
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F1-ad-7-5-604: The concentration of hydrogen peroxide in the peripheral blood of healthy volunteers (N = 34) and OSA patients (N = 43) and correlation with severity of OSA. Mean concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA patients (A). Mean concentration of hydrogen peroxide in the peripheral blood of OSA patients compared by severity of OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients) (B) and a significant correlation was observed between the concentration of hydrogen peroxide and the RDI of 43 OSA patients (R2=0.615) (C). Graphs show mean values. White dot: the concentration of hydrogen peroxide in healthy volunteers; black dot: the concentration of hydrogen peroxide in OSA patients (*p < .05 comparing healthy volunteers and OSA patients).

Mentions: To examine the burden of systemic oxidative stress in OSA patients, we compared the mean concentration of hydrogen peroxide in patients’ peripheral blood with that of healthy volunteers. The results showed that the mean hydrogen peroxide concentration in the peripheral blood of healthy volunteers (N=34) was 1.38 + 0.4 uM and the hydrogen peroxide concentration was significantly higher in peripheral blood of OSA patients (N=43, 2.29 ± 0.7 uM, p < 0.05) (Fig. 1A). We assessed the correlation between the hydrogen peroxide concentration in OSA patients and OSA severity. The results showed that the hydrogen peroxide concentration in the peripheral blood increased with greater disease severity. Patients with severe OSA had the highest hydrogen peroxide concentration in their peripheral blood (2.78 + 0.6 uM) compared with mild OSA (1.80 + 0.4 uM, p < 0.05, Fig. 1B). In addition, the hydrogen peroxide concentration of the peripheral blood of OSA patients was proportionally related to their RDI (R2=0.615, p < 0.05 Fig. 1C). We found that OSA patients might be exposed to relatively higher oxidative stress in their peripheral blood in accordance with severity of disease and apneic events.


Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea
The concentration of hydrogen peroxide in the peripheral blood of healthy volunteers (N = 34) and OSA patients (N = 43) and correlation with severity of OSA. Mean concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA patients (A). Mean concentration of hydrogen peroxide in the peripheral blood of OSA patients compared by severity of OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients) (B) and a significant correlation was observed between the concentration of hydrogen peroxide and the RDI of 43 OSA patients (R2=0.615) (C). Graphs show mean values. White dot: the concentration of hydrogen peroxide in healthy volunteers; black dot: the concentration of hydrogen peroxide in OSA patients (*p < .05 comparing healthy volunteers and OSA patients).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036955&req=5

F1-ad-7-5-604: The concentration of hydrogen peroxide in the peripheral blood of healthy volunteers (N = 34) and OSA patients (N = 43) and correlation with severity of OSA. Mean concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA patients (A). Mean concentration of hydrogen peroxide in the peripheral blood of OSA patients compared by severity of OSA (11 mild OSA (circle), 16 moderate OSA (rectangle), and 16 severe OSA (diamond) patients) (B) and a significant correlation was observed between the concentration of hydrogen peroxide and the RDI of 43 OSA patients (R2=0.615) (C). Graphs show mean values. White dot: the concentration of hydrogen peroxide in healthy volunteers; black dot: the concentration of hydrogen peroxide in OSA patients (*p < .05 comparing healthy volunteers and OSA patients).
Mentions: To examine the burden of systemic oxidative stress in OSA patients, we compared the mean concentration of hydrogen peroxide in patients’ peripheral blood with that of healthy volunteers. The results showed that the mean hydrogen peroxide concentration in the peripheral blood of healthy volunteers (N=34) was 1.38 + 0.4 uM and the hydrogen peroxide concentration was significantly higher in peripheral blood of OSA patients (N=43, 2.29 ± 0.7 uM, p < 0.05) (Fig. 1A). We assessed the correlation between the hydrogen peroxide concentration in OSA patients and OSA severity. The results showed that the hydrogen peroxide concentration in the peripheral blood increased with greater disease severity. Patients with severe OSA had the highest hydrogen peroxide concentration in their peripheral blood (2.78 + 0.6 uM) compared with mild OSA (1.80 + 0.4 uM, p < 0.05, Fig. 1B). In addition, the hydrogen peroxide concentration of the peripheral blood of OSA patients was proportionally related to their RDI (R2=0.615, p < 0.05 Fig. 1C). We found that OSA patients might be exposed to relatively higher oxidative stress in their peripheral blood in accordance with severity of disease and apneic events.

View Article: PubMed Central - PubMed

ABSTRACT

The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients.

No MeSH data available.