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BSN723T Prevents Atherosclerosis and Weight Gain in ApoE Knockout Mice Fed a Western Diet

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ABSTRACT

Objective: This study tests the hypothesis that BSN723T can prevent the development of hyperlipidemia and atherosclerosis in ApoE-/- knockout mice fed a Western (high fat, high cholesterol, and high sucrose) diet. BSN723T is a combination drug therapy consisting of D-tagatose and dihydromyricetin (BSN723).

Background: D-tagatose has an antihyperglycemic effect in animal and human studies and shows promise as a treatment for type 2 diabetes and obesity. Many claims regarding BSN723's pharmacological activities have been made including anti-cancer, anti-diabetic, anti-hypertensive, anti-inflammatory, and anti-atherosclerotic effects. To our knowledge this is the first study that combines D-tagatose and BSN723 for the treatment of hyperlipidemia and the prevention of atherosclerosis.

Methods: ApoE-deficient mice were randomized into five groups with equivalent mean body weights. The mice were given the following diets for 8 weeks: Group 1 - Standard diet; Group 2 - Western diet; Group 3 - Western diet formulated with D-tagatose; Group 4 - Western diet formulated with BSN723; Group 5 - Western diet formulated with BSN723T. Mice were measured for weight gain, tissue and organ weights, total serum cholesterol and triglycerides and formation of atherosclerosis.

Results: The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue and weight gain brought on by the Western diet. Both D-tagatose and BSN723 alone reduced total cholesterol and the formation of atherosclerosis in the aorta compared to mice on the Western diet. Addition of BSN723 to D-tagatose (BSN723T) did not increase efficacy in prevention of increases in cholesterol or atherosclerosis compared to D-tagatose alone.

Conclusion: Addition of either D-tagatose or BSN723 alone to a Western diet prevented weight gain, increases in total serum cholesterol and triglycerides, and the formation of atherosclerosis. However, there was no additive or synergistic effect on the measured parameters with the combination BSN723T treatment.

No MeSH data available.


Organ weights. A) Livers. B) Spleens. C) Lungs. D) Kidneys. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.
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Figure 7: Organ weights. A) Livers. B) Spleens. C) Lungs. D) Kidneys. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.

Mentions: Livers from mice on the Western diet weighed significantly more than livers from mice on all the other diets (P < 0.02) (Illustration 7A). There was no difference between livers of mice from any of the other groups. The only significant differences in the weights of spleens were from mice on the Western and BSN723 diets compared to spleens from mice on the Standard diet (P < 0.02) (Illustration 7B). There was no difference between spleens of mice from any of the other groups. The lungs from mice on the D-tagatose diet (Group 3) were slightly smaller (P = 0.012) than the lungs from the Standard diet fed mice (Illustration 7C). There were no differences between any of the other groups. Kidneys from mice that received D-tagatose in their diets (groups 3 and 5) were slightly smaller than kidneys from mice in the other groups (P < 0.02) (Illustration 7D).


BSN723T Prevents Atherosclerosis and Weight Gain in ApoE Knockout Mice Fed a Western Diet
Organ weights. A) Livers. B) Spleens. C) Lungs. D) Kidneys. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC5036941&req=5

Figure 7: Organ weights. A) Livers. B) Spleens. C) Lungs. D) Kidneys. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.
Mentions: Livers from mice on the Western diet weighed significantly more than livers from mice on all the other diets (P < 0.02) (Illustration 7A). There was no difference between livers of mice from any of the other groups. The only significant differences in the weights of spleens were from mice on the Western and BSN723 diets compared to spleens from mice on the Standard diet (P < 0.02) (Illustration 7B). There was no difference between spleens of mice from any of the other groups. The lungs from mice on the D-tagatose diet (Group 3) were slightly smaller (P = 0.012) than the lungs from the Standard diet fed mice (Illustration 7C). There were no differences between any of the other groups. Kidneys from mice that received D-tagatose in their diets (groups 3 and 5) were slightly smaller than kidneys from mice in the other groups (P < 0.02) (Illustration 7D).

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Objective: This study tests the hypothesis that BSN723T can prevent the development of hyperlipidemia and atherosclerosis in ApoE-/- knockout mice fed a Western (high fat, high cholesterol, and high sucrose) diet. BSN723T is a combination drug therapy consisting of D-tagatose and dihydromyricetin (BSN723).

Background: D-tagatose has an antihyperglycemic effect in animal and human studies and shows promise as a treatment for type 2 diabetes and obesity. Many claims regarding BSN723's pharmacological activities have been made including anti-cancer, anti-diabetic, anti-hypertensive, anti-inflammatory, and anti-atherosclerotic effects. To our knowledge this is the first study that combines D-tagatose and BSN723 for the treatment of hyperlipidemia and the prevention of atherosclerosis.

Methods: ApoE-deficient mice were randomized into five groups with equivalent mean body weights. The mice were given the following diets for 8 weeks: Group 1 - Standard diet; Group 2 - Western diet; Group 3 - Western diet formulated with D-tagatose; Group 4 - Western diet formulated with BSN723; Group 5 - Western diet formulated with BSN723T. Mice were measured for weight gain, tissue and organ weights, total serum cholesterol and triglycerides and formation of atherosclerosis.

Results: The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue and weight gain brought on by the Western diet. Both D-tagatose and BSN723 alone reduced total cholesterol and the formation of atherosclerosis in the aorta compared to mice on the Western diet. Addition of BSN723 to D-tagatose (BSN723T) did not increase efficacy in prevention of increases in cholesterol or atherosclerosis compared to D-tagatose alone.

Conclusion: Addition of either D-tagatose or BSN723 alone to a Western diet prevented weight gain, increases in total serum cholesterol and triglycerides, and the formation of atherosclerosis. However, there was no additive or synergistic effect on the measured parameters with the combination BSN723T treatment.

No MeSH data available.