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BSN723T Prevents Atherosclerosis and Weight Gain in ApoE Knockout Mice Fed a Western Diet

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ABSTRACT

Objective: This study tests the hypothesis that BSN723T can prevent the development of hyperlipidemia and atherosclerosis in ApoE-/- knockout mice fed a Western (high fat, high cholesterol, and high sucrose) diet. BSN723T is a combination drug therapy consisting of D-tagatose and dihydromyricetin (BSN723).

Background: D-tagatose has an antihyperglycemic effect in animal and human studies and shows promise as a treatment for type 2 diabetes and obesity. Many claims regarding BSN723's pharmacological activities have been made including anti-cancer, anti-diabetic, anti-hypertensive, anti-inflammatory, and anti-atherosclerotic effects. To our knowledge this is the first study that combines D-tagatose and BSN723 for the treatment of hyperlipidemia and the prevention of atherosclerosis.

Methods: ApoE-deficient mice were randomized into five groups with equivalent mean body weights. The mice were given the following diets for 8 weeks: Group 1 - Standard diet; Group 2 - Western diet; Group 3 - Western diet formulated with D-tagatose; Group 4 - Western diet formulated with BSN723; Group 5 - Western diet formulated with BSN723T. Mice were measured for weight gain, tissue and organ weights, total serum cholesterol and triglycerides and formation of atherosclerosis.

Results: The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue and weight gain brought on by the Western diet. Both D-tagatose and BSN723 alone reduced total cholesterol and the formation of atherosclerosis in the aorta compared to mice on the Western diet. Addition of BSN723 to D-tagatose (BSN723T) did not increase efficacy in prevention of increases in cholesterol or atherosclerosis compared to D-tagatose alone.

Conclusion: Addition of either D-tagatose or BSN723 alone to a Western diet prevented weight gain, increases in total serum cholesterol and triglycerides, and the formation of atherosclerosis. However, there was no additive or synergistic effect on the measured parameters with the combination BSN723T treatment.

No MeSH data available.


Adipose tissues from mice. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.
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Figure 6: Adipose tissues from mice. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.

Mentions: Mice on the Western diet had increased total adipose tissue (epidydimal + retroperitoneal + subcutaneous adipose tissue) compared to mice on the Standard diet (Illustration 6). The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue brought on by the Western diet and, in fact, mice consuming D-tagatose (Groups 3 and 5) were leaner than the mice on the Standard diet. Mice on the BSN723 diet were also leaner than those on the Western diet but were not significantly different from mice on the Standard diet. While mice on the BSN723 had more epididymal fat than mice on the Standard diet (0.67 ± 0.14 g vs 0.47 ± 0.09 g, respectively), the difference was not statistically significant (P = 0.26). The amount of subcutaneous (0.2 ± 0.03 g) and retroperitoneal fat (0.23 ± 0.06 g) in mice on the BSN723 mice was similar to that found in mice on the Standard diet (0.19 ± 0.04 g and 0.29 ± 0.07 g, respectively).


BSN723T Prevents Atherosclerosis and Weight Gain in ApoE Knockout Mice Fed a Western Diet
Adipose tissues from mice. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036941&req=5

Figure 6: Adipose tissues from mice. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10; BSN723 diet, n = 9; BSN723T diet, n = 10. Results are reported as mean +/- s.e.m.
Mentions: Mice on the Western diet had increased total adipose tissue (epidydimal + retroperitoneal + subcutaneous adipose tissue) compared to mice on the Standard diet (Illustration 6). The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue brought on by the Western diet and, in fact, mice consuming D-tagatose (Groups 3 and 5) were leaner than the mice on the Standard diet. Mice on the BSN723 diet were also leaner than those on the Western diet but were not significantly different from mice on the Standard diet. While mice on the BSN723 had more epididymal fat than mice on the Standard diet (0.67 ± 0.14 g vs 0.47 ± 0.09 g, respectively), the difference was not statistically significant (P = 0.26). The amount of subcutaneous (0.2 ± 0.03 g) and retroperitoneal fat (0.23 ± 0.06 g) in mice on the BSN723 mice was similar to that found in mice on the Standard diet (0.19 ± 0.04 g and 0.29 ± 0.07 g, respectively).

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Objective: This study tests the hypothesis that BSN723T can prevent the development of hyperlipidemia and atherosclerosis in ApoE-/- knockout mice fed a Western (high fat, high cholesterol, and high sucrose) diet. BSN723T is a combination drug therapy consisting of D-tagatose and dihydromyricetin (BSN723).

Background: D-tagatose has an antihyperglycemic effect in animal and human studies and shows promise as a treatment for type 2 diabetes and obesity. Many claims regarding BSN723's pharmacological activities have been made including anti-cancer, anti-diabetic, anti-hypertensive, anti-inflammatory, and anti-atherosclerotic effects. To our knowledge this is the first study that combines D-tagatose and BSN723 for the treatment of hyperlipidemia and the prevention of atherosclerosis.

Methods: ApoE-deficient mice were randomized into five groups with equivalent mean body weights. The mice were given the following diets for 8 weeks: Group 1 - Standard diet; Group 2 - Western diet; Group 3 - Western diet formulated with D-tagatose; Group 4 - Western diet formulated with BSN723; Group 5 - Western diet formulated with BSN723T. Mice were measured for weight gain, tissue and organ weights, total serum cholesterol and triglycerides and formation of atherosclerosis.

Results: The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue and weight gain brought on by the Western diet. Both D-tagatose and BSN723 alone reduced total cholesterol and the formation of atherosclerosis in the aorta compared to mice on the Western diet. Addition of BSN723 to D-tagatose (BSN723T) did not increase efficacy in prevention of increases in cholesterol or atherosclerosis compared to D-tagatose alone.

Conclusion: Addition of either D-tagatose or BSN723 alone to a Western diet prevented weight gain, increases in total serum cholesterol and triglycerides, and the formation of atherosclerosis. However, there was no additive or synergistic effect on the measured parameters with the combination BSN723T treatment.

No MeSH data available.