Limits...
BSN723T Prevents Atherosclerosis and Weight Gain in ApoE Knockout Mice Fed a Western Diet

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Objective: This study tests the hypothesis that BSN723T can prevent the development of hyperlipidemia and atherosclerosis in ApoE-/- knockout mice fed a Western (high fat, high cholesterol, and high sucrose) diet. BSN723T is a combination drug therapy consisting of D-tagatose and dihydromyricetin (BSN723).

Background: D-tagatose has an antihyperglycemic effect in animal and human studies and shows promise as a treatment for type 2 diabetes and obesity. Many claims regarding BSN723's pharmacological activities have been made including anti-cancer, anti-diabetic, anti-hypertensive, anti-inflammatory, and anti-atherosclerotic effects. To our knowledge this is the first study that combines D-tagatose and BSN723 for the treatment of hyperlipidemia and the prevention of atherosclerosis.

Methods: ApoE-deficient mice were randomized into five groups with equivalent mean body weights. The mice were given the following diets for 8 weeks: Group 1 - Standard diet; Group 2 - Western diet; Group 3 - Western diet formulated with D-tagatose; Group 4 - Western diet formulated with BSN723; Group 5 - Western diet formulated with BSN723T. Mice were measured for weight gain, tissue and organ weights, total serum cholesterol and triglycerides and formation of atherosclerosis.

Results: The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue and weight gain brought on by the Western diet. Both D-tagatose and BSN723 alone reduced total cholesterol and the formation of atherosclerosis in the aorta compared to mice on the Western diet. Addition of BSN723 to D-tagatose (BSN723T) did not increase efficacy in prevention of increases in cholesterol or atherosclerosis compared to D-tagatose alone.

Conclusion: Addition of either D-tagatose or BSN723 alone to a Western diet prevented weight gain, increases in total serum cholesterol and triglycerides, and the formation of atherosclerosis. However, there was no additive or synergistic effect on the measured parameters with the combination BSN723T treatment.

No MeSH data available.


Related in: MedlinePlus

Comparison of the percent area of the aortic arch that has plaque. Aortas were prepared for atherosclerosis measurements via en face presentation. The addition of D-tagatose, dihydromyricetin or BSN723T to the Western diet significantly prevented the formation of atherosclerotic plaques. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10, BSN723, n = 9, BSN723T, n = 10. Results are shown as mean +/- s.e.m.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5036941&req=5

Figure 10: Comparison of the percent area of the aortic arch that has plaque. Aortas were prepared for atherosclerosis measurements via en face presentation. The addition of D-tagatose, dihydromyricetin or BSN723T to the Western diet significantly prevented the formation of atherosclerotic plaques. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10, BSN723, n = 9, BSN723T, n = 10. Results are shown as mean +/- s.e.m.

Mentions: The surface area of aortas covered by atherosclerotic lesion was greater in mice on the Western diet compared to mice on the Standard diet (P = 0.001) (Illustration 10). The addition of D-tagatose, BSN723, or BSB723T to the diets inhibited the formation of plaque in aortas compared to mice on the Western diet (P = 0.007, P = 0.04, P = 0.016, respectively). The aortas from mice that were on the D-tagatose, BSN723, and BSN723T diets all had greater plaque formation compared to mice on the Standard diet (P = 0.023, P < 0.0001, P = 0.02, respectively). Significant differences were found between mice from Group 1 (Standard diet) and all other groups (P < 0.022), between Group 2 (Western diet) and all other groups (P < 0.04), and between Groups 3 (D-tagatose diet) and 4 (BSN723 diet) (P = 0.014).


BSN723T Prevents Atherosclerosis and Weight Gain in ApoE Knockout Mice Fed a Western Diet
Comparison of the percent area of the aortic arch that has plaque. Aortas were prepared for atherosclerosis measurements via en face presentation. The addition of D-tagatose, dihydromyricetin or BSN723T to the Western diet significantly prevented the formation of atherosclerotic plaques. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10, BSN723, n = 9, BSN723T, n = 10. Results are shown as mean +/- s.e.m.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5036941&req=5

Figure 10: Comparison of the percent area of the aortic arch that has plaque. Aortas were prepared for atherosclerosis measurements via en face presentation. The addition of D-tagatose, dihydromyricetin or BSN723T to the Western diet significantly prevented the formation of atherosclerotic plaques. Standard diet, n = 9; Western diet, n = 10; D-tagatose diet, n = 10, BSN723, n = 9, BSN723T, n = 10. Results are shown as mean +/- s.e.m.
Mentions: The surface area of aortas covered by atherosclerotic lesion was greater in mice on the Western diet compared to mice on the Standard diet (P = 0.001) (Illustration 10). The addition of D-tagatose, BSN723, or BSB723T to the diets inhibited the formation of plaque in aortas compared to mice on the Western diet (P = 0.007, P = 0.04, P = 0.016, respectively). The aortas from mice that were on the D-tagatose, BSN723, and BSN723T diets all had greater plaque formation compared to mice on the Standard diet (P = 0.023, P < 0.0001, P = 0.02, respectively). Significant differences were found between mice from Group 1 (Standard diet) and all other groups (P < 0.022), between Group 2 (Western diet) and all other groups (P < 0.04), and between Groups 3 (D-tagatose diet) and 4 (BSN723 diet) (P = 0.014).

View Article: PubMed Central - HTML - PubMed

ABSTRACT

Objective: This study tests the hypothesis that BSN723T can prevent the development of hyperlipidemia and atherosclerosis in ApoE-/- knockout mice fed a Western (high fat, high cholesterol, and high sucrose) diet. BSN723T is a combination drug therapy consisting of D-tagatose and dihydromyricetin (BSN723).

Background: D-tagatose has an antihyperglycemic effect in animal and human studies and shows promise as a treatment for type 2 diabetes and obesity. Many claims regarding BSN723's pharmacological activities have been made including anti-cancer, anti-diabetic, anti-hypertensive, anti-inflammatory, and anti-atherosclerotic effects. To our knowledge this is the first study that combines D-tagatose and BSN723 for the treatment of hyperlipidemia and the prevention of atherosclerosis.

Methods: ApoE-deficient mice were randomized into five groups with equivalent mean body weights. The mice were given the following diets for 8 weeks: Group 1 - Standard diet; Group 2 - Western diet; Group 3 - Western diet formulated with D-tagatose; Group 4 - Western diet formulated with BSN723; Group 5 - Western diet formulated with BSN723T. Mice were measured for weight gain, tissue and organ weights, total serum cholesterol and triglycerides and formation of atherosclerosis.

Results: The addition of D-tagatose, either alone or in combination with BSN723, prevented the increase in adipose tissue and weight gain brought on by the Western diet. Both D-tagatose and BSN723 alone reduced total cholesterol and the formation of atherosclerosis in the aorta compared to mice on the Western diet. Addition of BSN723 to D-tagatose (BSN723T) did not increase efficacy in prevention of increases in cholesterol or atherosclerosis compared to D-tagatose alone.

Conclusion: Addition of either D-tagatose or BSN723 alone to a Western diet prevented weight gain, increases in total serum cholesterol and triglycerides, and the formation of atherosclerosis. However, there was no additive or synergistic effect on the measured parameters with the combination BSN723T treatment.

No MeSH data available.


Related in: MedlinePlus