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Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust

View Article: PubMed Central - PubMed

ABSTRACT

Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS.1BL translocation chromosomes in the other 3 translocation lines, which have been named RT1163-4, RT1217-1, and RT1249. When inoculated with stripe rust isolates, these 4 lines expressed high resistance to several Puccinia striiformis f. sp Tritici pathotypes that are virulent on Yr9. Moreover, the different response pattern of resistance among them suggested that the diversity of resistance genes for wheat stripe rust exists in the rye. These 4 lines also showed better agronomic performances than their wheat parent. The GS indices also showed the genetic diversity of the 1RS which derived from same rye variety. The present study indicates that rye cultivars may carry untapped variations that could potentially be used for wheat improvement.

No MeSH data available.


PCR results of 4 specific primers: O11B3 and O11B5, ω-sec-P1 and ω-sec-P2.Lane 1 = RT1249; lane 2 = RT1163-4; lane 3 = RT1217-11; lane 4 = RS1200-3; lane 5 = CN11; lane 6 = MY11; lane 7 = Aigan rye; lane M = marker.
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pone.0163642.g002: PCR results of 4 specific primers: O11B3 and O11B5, ω-sec-P1 and ω-sec-P2.Lane 1 = RT1249; lane 2 = RT1163-4; lane 3 = RT1217-11; lane 4 = RS1200-3; lane 5 = CN11; lane 6 = MY11; lane 7 = Aigan rye; lane M = marker.

Mentions: Molecular markers were also used for identify these wheat translocation/substitution lines. Primer pairs O11B3 and O11B5 are the specific primers of 1BS, and a 630 bp fragment would be amplified by them. Another primer pair, ω-sec-P1 and ω-sec-P2, are the specific primers of 1RS, and they can amplified a 1076 bp fragment. The PCR results showed that only wheat cultivar MY11 amplified a 630 bp band. Both the translocation lines and the substitution line can amplify a 1076 bp band but no 630 bp band was amplified (Fig 2).


Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust
PCR results of 4 specific primers: O11B3 and O11B5, ω-sec-P1 and ω-sec-P2.Lane 1 = RT1249; lane 2 = RT1163-4; lane 3 = RT1217-11; lane 4 = RS1200-3; lane 5 = CN11; lane 6 = MY11; lane 7 = Aigan rye; lane M = marker.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5036889&req=5

pone.0163642.g002: PCR results of 4 specific primers: O11B3 and O11B5, ω-sec-P1 and ω-sec-P2.Lane 1 = RT1249; lane 2 = RT1163-4; lane 3 = RT1217-11; lane 4 = RS1200-3; lane 5 = CN11; lane 6 = MY11; lane 7 = Aigan rye; lane M = marker.
Mentions: Molecular markers were also used for identify these wheat translocation/substitution lines. Primer pairs O11B3 and O11B5 are the specific primers of 1BS, and a 630 bp fragment would be amplified by them. Another primer pair, ω-sec-P1 and ω-sec-P2, are the specific primers of 1RS, and they can amplified a 1076 bp fragment. The PCR results showed that only wheat cultivar MY11 amplified a 630 bp band. Both the translocation lines and the substitution line can amplify a 1076 bp band but no 630 bp band was amplified (Fig 2).

View Article: PubMed Central - PubMed

ABSTRACT

Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS.1BL translocation chromosomes in the other 3 translocation lines, which have been named RT1163-4, RT1217-1, and RT1249. When inoculated with stripe rust isolates, these 4 lines expressed high resistance to several Puccinia striiformis f. sp Tritici pathotypes that are virulent on Yr9. Moreover, the different response pattern of resistance among them suggested that the diversity of resistance genes for wheat stripe rust exists in the rye. These 4 lines also showed better agronomic performances than their wheat parent. The GS indices also showed the genetic diversity of the 1RS which derived from same rye variety. The present study indicates that rye cultivars may carry untapped variations that could potentially be used for wheat improvement.

No MeSH data available.